Formation of cyclic deoxyguanosine adducts in Chinese hamster ovary cells by acrolein and crotonaldehyde

Autor:	Lisa M. Miglietta, Shobha A. Akerkar, Fung-Lung Chung, Peter G. Foiles
Rok vydání:	1990
Předmět:	Aldehydes Cancer Research Mutagenicity Tests Chemistry Chinese hamster ovary cell Acrolein Deoxyguanosine Hamster Mutagen DNA General Medicine medicine.disease_cause Adduct chemistry.chemical_compound Biochemistry Cricetinae medicine Animals Crotonaldehyde Cells Cultured Carcinogen
Zdroj:	Carcinogenesis. 11:2059-2061
ISSN:	1460-2180 0143-3334
DOI:	10.1093/carcin/11.11.2059
Popis:	Acrolein and crotonaldehyde are alpha,beta-unsaturated carbonyl compounds that form 1,N2-propanodeoxyguanosine adducts when reacted with DNA in vitro. These compounds are mutagenic in Salmonella, and crotonaldehyde is tumorigenic in rats. This study used immunoassay and 32P-postlabeling methods to determine if acrolein and crotonaldehyde form these adducts in cultured mammalian cells. Adduct levels were highest in Chinese hamster ovary cells exposed to acrolein (1 mM) with 162 mumol adduct/mol deoxyguanosine. Crotonaldehyde (10 mM) formed adduct at a level of 75 mumol/mol deoxyguanosine. 32P-Postlabeling analysis confirmed the presence of adducts in crotonaldehyde-treated cells. Persistence studies showed that adduct levels were unchanged if the cells were cultured for 6 h before DNA isolation. Mutagenicity studies were performed to determine the biological consequences of these adducts. Mutations were not observed due to the toxicity of the compounds.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7be852b7b0bd50b4a74ebd80bfe5d4b https://doi.org/10.1093/carcin/11.11.2059 Zobrazit plný text záznamu