Differences in expression of genes in the MyD88 and TRIF signalling pathways and methylation of TLR4 and TRIF in Tibetan chickens and DaHeng S03 chickens infected with Salmonella enterica serovar enteritidis
| Autor: | Wei Jiang, Chaowu Yang, Zeng-Rong Zhang, Huarui Du, Shuai Men, Hongning Wang, Zhikun Zhang, Xiaocheng Li, Peng Zhang, Xiaosong Jiang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Salmonella animal structures Salmonella enteritidis Immunology Biology medicine.disease_cause Microbiology 03 medical and health sciences 0302 clinical medicine medicine Animals Gene Poultry Diseases Salmonella Infections Animal General Veterinary Methylation DNA Methylation Acquired immune system biology.organism_classification Toll-Like Receptor 4 Adaptor Proteins Vesicular Transport 030104 developmental biology Gene Expression Regulation TRIF Salmonella enterica Myeloid Differentiation Factor 88 TLR4 Female Disease Susceptibility Chickens 030215 immunology Signal Transduction |
| Zdroj: | Veterinary immunology and immunopathology. 189 |
| ISSN: | 1873-2534 |
| Popis: | Salmonella enterica serovar (S. enteritidis) is a pathogenic bacterium that can cause symptoms of food poisoning, leading to death of poultry, resulting in serious economic losses. The MyD88 and TRIF signalling pathways play important roles in activating innate and adaptive immunity in chickens infected with S. enteritidis. The objective of the present study was to characterize in vivo mRNA expressions, protein levels and methylation levels of genes in the above two pathways in both Tibetan chickens and DaHeng S03 chickens infected with S. enteritidis. MyD88-dependent and TRIF-dependent signalling pathway were activated by infection, and the MyD88 signalling pathway induced cytokines LITAF and IL-8 played important roles in fighting against the S. enteritidis infection in vivo. The TLR4 methylation might alter expression of genes involved in the MyD88 signalling pathway, and thus different breeds of chickens might show differences in susceptibility to the S. enteritidis. The increased expression of INF β was activated by S. enteritidis, but its expressions were different in levels of mRNA and protein in Tibetan chickens and DaHeng chickens, suggesting its functions on the resistance to S. enteritidis infection in chickens. This study contributes to the understanding of two pathways activated in response to S. enteritidis infection, and gives indications on the mechanisms underlying resistance of Tibetan chickens and DaHeng chickens to S. enteritidis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect