The nucleolar localisation signal of the HTLV-I protein p27rex is important for stabilisation of IL-2 receptor α subunit mRNA by p27rex
| Autor: | Tetsuya Nosaka, Tasuku Honjo, Hiroshi Kanamori, Kenneth N White, Masakazu Hatanaka |
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| Rok vydání: | 1991 |
| Předmět: |
Macromolecular Substances
Nucleolus viruses Mutant Biophysics Fluorescent Antibody Technique Biology Transfection Biochemistry Cell Line Cell surface receptor Animals RNA Messenger Molecular Biology G alpha subunit Human T-lymphotropic virus 1 Messenger RNA Nucleoplasm Genes pX Receptors Interleukin-2 Cell Biology Molecular biology Gene Products rex Cytoplasm Signal transduction Cell Nucleolus Plasmids Signal Transduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 175:98-103 |
| ISSN: | 0006-291X |
| Popis: | In this study we investigated the mechanism of stabilisation of IL-2 receptor alpha subunit mRNA by the HTLV-I protein p27rex. We tested the role of the nucleolar targetting signal in rex by introducing mutations. Three deletion mutants could not express rex protein in the nucleolus and although protein was still expressed in the nucleoplasm none of the mutants could stabilise IL-2R alpha mRNA. A substitution mutant could be expressed in the nucleolus and could also stabilise IL-2R alpha mRNA. The data show that the nucleolar targetting signal is crucial for stabilisation of IL-2R alpha mRNA by rex and raise the possibility that transport of mRNA from nucleus to cytoplasm can involve the nucleolus. |
| Databáze: | OpenAIRE |
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