Immunohistochemical analysis of thrombomodulin expression in myocardial tissue from autopsy cases of ischemic heart disease
| Autor: | Azumi Kuse, Mai Morichika, Kanako Nakagawa, Migiwa Asano, Yasuhiro Ueno, Makoto Sakurada, Takeshi Kondo, Gentaro Yamasaki, Marie Sugimoto, Motonori Takahashi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty Thrombomodulin Myocardial Ischemia Autopsy 01 natural sciences Myocardial remodeling Pathology and Forensic Medicine Endothelial injury Forensic pathology Mice 03 medical and health sciences 0302 clinical medicine Thrombin Downregulation and upregulation medicine Animals 030216 legal & forensic medicine Myocardial infarction business.industry Myocardium 010401 analytical chemistry Endothelial Cells medicine.disease Immunohistochemistry 0104 chemical sciences Issues ethics and legal aspects Coagulation cardiovascular system business Immunostaining medicine.drug |
| Zdroj: | Legal Medicine. 51:101897 |
| ISSN: | 1344-6223 |
| Popis: | Thrombomodulin is a transmembrane glycoprotein that is ubiquitously expressed on the surface of vascular endothelial cells. Thrombomodulin exerts its anticoagulant effects by combining with thrombin, activating protein C, and inactivating the coagulation factors FVa and FVIIIa. Clinically, thrombomodulin is also known as a marker of vascular injury because it circulates freely in response to endothelial injury. In this study, myocardial tissue from cases of ischemic heart disease was subjected to immunohistochemistry by thrombomodulin. We examined 40 neutral-formalin-fixed, paraffin-embedded myocardial tissue samples from autopsy cases that were diagnosed with ischemic heart disease (within 48 h postmortem). Thrombomodulin expression was observed in vascular endothelial cells between myocardial cells and in mesothelial cells of the epicardium. In necrotic myocardium, diffusion of thrombomodulin, which reflected endothelial injury, was observed. Upregulated thrombomodulin expression was observed around myocardial cells under ongoing remodeling, which suggested endothelial proliferation in these locations. Completed fibrotic foci of the myocardium did not show upregulated thrombomodulin expression. In a mouse model of acute myocardial infarction, the same phenomena as that found in human samples were observed by immunohistochemistry of thrombomodulin. Immunostaining of thrombomodulin, as a marker for endothelial injury or myocardial remodeling, may be useful for supplementing conventional staining techniques in the diagnosis of ischemic heart disease in forensic pathology. |
| Databáze: | OpenAIRE |
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