Rapid suppression of protein degradation in skeletal muscle after oral feeding of leucine in rats
| Autor: | Takashi Nagasawa, Naoyuki Nishizawa, Yoshiaki Ito, Fumiaki Yoshizawa, Taketoshi Kido |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Nutrition and Dietetics Endocrinology Diabetes and Metabolism Clinical Biochemistry Skeletal muscle Biology Protein degradation musculoskeletal system Biochemistry Extensor digitorum muscle Extensor digitorum longus muscle Postprandial medicine.anatomical_structure Endocrinology Casein Internal medicine medicine Leucine Myofibril tissues Molecular Biology |
| Zdroj: | The Journal of Nutritional Biochemistry. 13:121-127 |
| ISSN: | 0955-2863 |
| DOI: | 10.1016/s0955-2863(01)00209-1 |
| Popis: | A diet containing adequate amounts of protein rapidly suppresses myofibrillar protein degradation in rats and mice. This study determined whether dietary amino acids inhibit postprandial protein degradation in rat skeletal muscle. When rats fed on a 20% casein diet for 1 h after 18 h starvation, the rate of myofibrillar protein degradation measured by N(tau)-methylhistidine release from the isolated extensor digitorum longus muscle was significantly (p0.05) decreased at 4 h after refeeding. A diet containing an amino acid mixture which is the same composition as casein also reduced myofibrillar protein degradation at 4 h after refeeding (p0.05). An essential amino acid mixture (15.1%, corresponding to casein composition) and a leucine (2.9%) diets reduced the rate of myofibrillar protein degradation after refeeding (p0.05), whereas a protein free diet did not. Administration of leucine alone (0.135 g/100 g body weight) by a feeding tube induced a decrease in the rate of myofibrillar protein degradation at 2 h after administration (p0.05), whereas the serum insulin concentration was constant after leucine administration. These results suggested that leucine is one of regulating factors of myofibrillar protein degradation after refeeding of a protein diet. |
| Databáze: | OpenAIRE |
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