Analysis of 136 pesticides in avocado using a modified QuEChERS method with LC-MS/MS and GC-MS/MS
| Autor: | Sookwang Lee, Tiffany Harmon, Greg Mercer, Narong Chamkasem, Lisa W Ollis |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Chromatography
Molecular Structure Chemistry Persea Extraction (chemistry) Solid Phase Extraction Pesticide Residues Food Contamination General Chemistry Pesticide Tandem mass spectrometry Quechers Gas Chromatography-Mass Spectrometry Triple quadrupole mass spectrometer Tandem Mass Spectrometry Sample preparation Gas chromatography Gas chromatography–mass spectrometry General Agricultural and Biological Sciences Chromatography High Pressure Liquid |
| Zdroj: | Journal of agricultural and food chemistry. 61(10) |
| ISSN: | 1520-5118 |
| Popis: | A simple and high-throughput screening method for the analysis of 136 pesticides in avocado ( Persea americana ) by LC-(+)-ESI-MS/MS and GC-MS/MS is presented. A modified QuEChERS sample preparation method was developed to improve the extraction recovery of highly lipophilic pesticides. Extracts from minced avocados after acetonitrile (MeCN) extraction were directly injected to LC-MS/MS, whereas other GC-amenable compounds were treated with the modified QuEChERS procedure for GC-MS/MS analysis. The average recoveries for 79 pesticides quantified by LC-MS/MS at 10, 50, and 200 ng/g fortifying levels were 86.1% or better (with maximum RSD at 9.2%), whereas GC-MS/MS analysis demonstrated 70.2% or better (RSD < 18%) for average recovery from 57 compounds at the same spike levels. The application of LC- and GC-MS/MS combined with the improved extraction procedures led to the current method, which can quantitate these pesticides even if they are present in avocados below the targeted action level by FDA. This method demonstrated the improved recovery of several challenging lipophilic pesticides in highly fat-rich avocados. |
| Databáze: | OpenAIRE |
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