Conjugative plasmid mediated inducible nickel resistance in Hafnia alvei 5-5
| Autor: | H G Schlegel, Ho Sa Lee, Jeong Eun Park, Kho Eun Young, Ho Gun Rhie |
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| Rok vydání: | 2003 |
| Předmět: |
inorganic chemicals
Microbiology (medical) EcoRI chemistry.chemical_element Microbial Sensitivity Tests medicine.disease_cause Microbiology Plasmid Nickel Sequence Homology Nucleic Acid Drug Resistance Bacterial medicine Cloning Molecular Escherichia coli Bacteria Models Genetic biology Structural gene Hafnia alvei Klebsiella oxytoca biology.organism_classification Molecular biology chemistry Conjugation Genetic Mutation Serratia marcescens biology.protein DNA Probes Hybrid plasmid Plasmids |
| Zdroj: | International Microbiology. 6:57-64 |
| ISSN: | 1618-1905 1139-6709 |
| DOI: | 10.1007/s10123-003-0101-8 |
| Popis: | Hafnia alvei 5-5, isolated from a soil-litter mixture underneath the canopy of the nickel-hyperaccumulating tree Sebertia acuminata (Sapotaceae) in New Caledonia, was found to be resistant to 30 mM Ni(2+) or 2 mM Co(2+). The 70-kb plasmid, pEJH 501, was transferred by conjugation to Escherichia coli, Serratia marcescens, and Klebsiella oxytoca. Transconjugant strains expressed inducible nickel resistance to between 5 and 17 mM Ni(2+), and cobalt resistance to 2 mM Co(2+). A 4.8-kb Sal- EcoRI fragment containing the nickel resistance determinant was subcloned, and the hybrid plasmid was found to confer a moderate level of resistance to nickel (7 mM Ni(2+)) even to E. coli. The expression of nickel resistance was inducible by exposure to nickel chloride at a concentration as low as 0.5 mM Ni(2+). By random Tn phoA'-1 insertion mutagenesis, the fragment was shown to have structural genes as well as regulatory regions for nickel resistance. Southern hybridization studies showed that the nickel-resistance determinant from pEJH501 of H. alvei 5-5 was homologous to that of pTOM9 from Alcaligenes xylosoxydans 31A. |
| Databáze: | OpenAIRE |
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