Damaged DNA binding protein 1 in Drosophila defense reactions
| Autor: | Kei Ichi Takata, Gen Ishikawa, Ryo Takeuchi, Yoko Abe, Kaori Shimanouchi, Yoshihiro Kanai, Shizuka Murakami, Tatsushi Ruike, Ryou Ichi Nakamura, Masamitsu Yamaguchi, Kengo Sakaguchi |
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| Rok vydání: | 2004 |
| Předmět: |
Hemocytes
Transgene Antimicrobial peptides Biophysics Biology Biochemistry Animals Genetically Modified Immune system RNA interference Transcription (biology) Gene silencing Animals Drosophila Proteins Tissue Distribution Molecular Biology Endodeoxyribonucleases Leukemia fungi Cell Biology Molecular biology Immunity Innate Recombinant Proteins Damaged DNA binding DNA-Binding Proteins RNA silencing Organ Specificity Drosophila |
| Zdroj: | Biochemical and biophysical research communications. 323(3) |
| ISSN: | 0006-291X |
| Popis: | We have focused attention on functions of Drosophila damaged DNA binding protein 1 (D-DDB1) in Drosophila hematopoiesis and previously reported that its whole body dsRNA over-expression using a GAL4-UAS targeted expression system results in melanotic tumors and complete lethality. Since the lesions appear to arise as a normal and heritable response to abnormal development, forming groups of cells that are recognized by the immune system and encapsulated in melanized cuticle, D-DDB1 appears to be an essential development-associated factor in Drosophila. To probe the possibility that it contributes to hemocyte development, we used a collagen promoter-GAL4 strain to over-express dsRNA of D-DDB1 in Drosophila hemocytes. The D-DDB1 gene silencing caused melanotic tumors and mortality at the end of larval development. Similarly, it interfered with melanization and synthesis of antimicrobial peptides. Transgenic flies with D-DDB1 gene silencing were found to accumulate abnormal large blood cells, reminiscent of human leukemia, suggesting that D-DDB1 has functions in hemocyte development. |
| Databáze: | OpenAIRE |
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