Two-dimensional cryostat section electrophoresis: a novel method and its application to the diseased synovial membrane
| Autor: | Anette Mischlinski, Johannes G. Saal, Cornelia Wicherek, Peter Fritz |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Pathology
medicine.medical_specialty Isoelectric focusing Chemistry Synovial Membrane Arthritis Proteins Cell Biology medicine.disease Silver stain Arthritis Rheumatoid Electrophoresis medicine.anatomical_structure Rheumatoid arthritis Synovitis medicine Frozen Sections Humans Electrophoresis Gel Two-Dimensional Anatomy Synovial membrane Polyacrylamide gel electrophoresis |
| Zdroj: | The Histochemical journal. 26(10) |
| ISSN: | 0018-2214 |
| Popis: | A two-dimensional electrophoresis technique for analysing sections of human tissue is described. Cryostat sections, 10 microns thick, are placed on an isoelectric focusing gel and then transferred to an SDS gel in the second dimension. The protein pattern is visualized by silver staining and is thought to represent soluble proteins. The silver-stained proteins were found to be both reproducible and, to the extent tested, organ-specific. This method was used to analyse 43 synovial membranes from patients suffering from rheumatoid arthritis or degenerative joint diseases. The analysis did not reveal any specific protein pattern for rheumatoid arthritis. The protein spot number was not related to the cause of arthritis. However, the total protein spot number was related to the histomorphological synovitis type, with those exhibiting either an exudative or proliferative synovitis pattern possessing significantly higher protein spot numbers than those specimens exhibiting a sero-fibrous or lympho-plasmacytic pattern of synovitis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink