Role of the Indigenous Microbiota in Maintaining the Virus-Specific CD8 Memory T Cells in the Lung of Mice Infected with Murine Cytomegalovirus
| Autor: | Tadayuki Satoh, Satoshi Noda, Sadaaki Sawamura, Kiyoshi Kobayashi, Kazuo Tanaka |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Muromegalovirus
Immunology CD8-Positive T-Lymphocytes Virus Epitope Interferon-gamma Mice Antigen Animals Immunology and Allergy Cytotoxic T cell Lung Mice Inbred BALB C biology CD44 Herpesviridae Infections biology.organism_classification Virology Molecular biology Specific Pathogen-Free Organisms Intestines biology.protein Cytokines Female Immunologic Memory Viral load CD8 |
| Zdroj: | The Journal of Immunology. 178:5209-5216 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.178.8.5209 |
| Popis: | The potent role of indigenous microbiota in maintaining murine CMV (MCMV)-specific memory T cells, which were measured by multimer staining, was investigated using germfree (GF) mice. When the BALB/c mice bred under specific pathogen-free (SPF) conditions were i.p. infected with 0.2 LD50 of MCMV, high frequencies of CD69+/CD44+ MCMV-specific CD8 T cells were noted in the lungs even at 6–12 mo after infection (11.1 ± 3.2 and 9.8 ± 0.9%, respectively). In contrast, even though the viral load and expression levels of mRNA of such cytokines as IL-2, IL-7, IL-15, and IFN-γ in the lungs of MCMV-infected GF mice were comparable to those of infected SPF mice, the frequencies of MCMV-specific CD8 T cells in the lungs of infected GF mice were kept lower than 1% at 6–12 mo after infection. In addition, the reconstitution of microbiota of MCMV-infected GF mice by orally administering a fecal suspension prepared from SPF mice restored the frequencies of both CD8+/multimer+ and CD8+/multimer− T cells to levels similar to those found in SPF mice. These results suggested the indigenous microbiota to play a crucial role in the expansion and maintenance of viral-specific CD8 memory T cells, probably by cross-reactivity between the antigenic epitope of the MCMV-specific memory T cells and the variety of peptides derived from the members of the microbiota. Such cross-reactivity may thus be a major feature of those cells. |
| Databáze: | OpenAIRE |
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