Ayanin diacetate-induced cell death is amplified by TRAIL in human leukemia cells
| Autor: | Mariana López, Sara Estévez, Jorge Triana, Francisco Estévez, Francisco León, José Quintana, Francisco Javier Montero Pérez, Gledy Negrín, María Teresa Marrero |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Programmed cell death
Ayanin Cell Survival Poly ADP ribose polymerase Biophysics Antineoplastic Agents Apoptosis HL-60 Cells Biochemistry TNF-Related Apoptosis-Inducing Ligand chemistry.chemical_compound Humans Molecular Biology Caspase Flavonoids Leukemia biology Cell growth Cytochrome c U937 Cells Cell Biology Cell cycle Flavones Molecular biology Up-Regulation G2 Phase Cell Cycle Checkpoints Receptors TNF-Related Apoptosis-Inducing Ligand chemistry Caspases biology.protein |
| Zdroj: | Biochemical and Biophysical Research Communications. 428:116-120 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2012.10.017 |
| Popis: | Here we demonstrate that the semi-synthetic flavonoid ayanin diacetate induces cell death selectively in leukemia cells without affecting the proliferation of normal lymphocytes. Incubation of human leukemia cells with ayanin diacetate induced G 2 -M phase cell cycle arrest and apoptosis which was prevented by the non-specific caspase inhibitor z-VAD-fmk and reduced by the overexpression of Bcl-x L . Ayanin diacetate-induced cell death was found to be associated with: (i) loss of inner mitochondrial membrane potential, (ii) the release of cytochrome c , (iii) the activation of multiple caspases, (iv) cleavage of poly(ADP-ribose) polymerase and (v) the up-regulation of death receptors for TRAIL, DR4 and DR5. Moreover, the combined treatment with ayanin diacetate and TRAIL amplified cell death, compared to single treatments. These results provide a basis for further exploring the potential applications of this combination for the treatment of cancer. |
| Databáze: | OpenAIRE |
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