Human herpesvirus type 1 and type 2 disrupt mitochondrial dynamics in human keratinocytes
| Autor: | Piotr Bąska, A. Słońska, M. W. Bańbura, Piotr Orlowski, A. Golke, Joanna Brzezicka, J. Cymerys, Malgorzata Krzyzowska, Izabela Serafińska, Marcin Chodkowski |
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| Rok vydání: | 2018 |
| Předmět: |
Keratinocytes
0301 basic medicine Herpesvirus 2 Human viruses Cell Herpesvirus 1 Human Mitochondrion Biology Mitochondrial Dynamics 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Movement Virology Organelle medicine Humans Antigens Viral Cell Line Transformed virus diseases Herpes Simplex General Medicine biochemical phenomena metabolism and nutrition Mitochondria Cell biology HaCaT 030104 developmental biology medicine.anatomical_structure chemistry Cytoplasm Cell culture DNA Viral Original Article 030217 neurology & neurosurgery DNA Homeostasis |
| Zdroj: | Archives of Virology |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/s00705-018-3890-y |
| Popis: | Mitochondrial movement and distribution throughout the cytoplasm is crucial for maintaining cell homeostasis. Mitochondria are dynamic organelles but can be functionally disrupted during infection. Here, we show that the ubiquitous human pathogens HHV-1 and HHV-2 induce changes in the mitochondrial morphology and distribution in the early and late phases of productive infection in human keratinocytes (HaCaT cells). We observed a decrease in the mitochondrial potential at 2 h postinfection and a decrease in cell vitality at 24 h postinfection. Moreover, we found that mitochondria migrated to the perinuclear area, where HHV-1 and HHV-2 antigens were also observed, mainly in the early stages of infection. Positive results of real-time PCR showed a high level of HHV-1 and HHV-2 DNA in HaCaT cells and culture medium. Our data demonstrate that HHV-1 and HHV-2 cause mitochondrial dysfunction in human keratinocytes. |
| Databáze: | OpenAIRE |
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