BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal Damage
| Autor: | Ajay Kale, Anne L. Spieles-Engemann, Petri Olivius, Samuel I. Stupp, Ekaterina Novozhilova, Björn Palmgren, Ulrica Englund Johansson, Yu Jiao |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Neurite Article Subject Cell Survival Green Fluorescent Proteins Nanofibers lcsh:Medicine Cell Count General Biochemistry Genetics and Molecular Biology Cochlear nucleus Rats Sprague-Dawley Neural Stem Cells Precursor cell medicine Neurotoxin Animals Humans Cochlear Nerve Brain-derived neurotrophic factor Neurons General Immunology and Microbiology business.industry Regeneration (biology) Brain-Derived Neurotrophic Factor lcsh:R Klinisk medicin Cell Differentiation General Medicine Cell biology Transplantation Disease Models Animal Ophthalmology Female Stem cell Clinical Medicine business Gels Research Article Stem Cell Transplantation |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2014 (2014) BioMed Research International; 2014, no 356415 (2014) |
| ISSN: | 2314-6133 |
| Popis: | Objectives. To study possible nerve regeneration of a damaged auditory nerve by the use of stem cell transplantation.Methods. We transplanted HNPCs to the rat AN trunk by the internal auditory meatus (IAM). Furthermore, we studied if addition of BDNF affects survival and phenotypic differentiation of the grafted HNPCs. A bioactive nanofiber gel (PA gel), in selected groups mixed with BDNF, was applied close to the implanted cells. Before transplantation, all rats had been deafened by a round window niche application ofβ-bungarotoxin. This neurotoxin causes a selective toxic destruction of the AN while keeping the hair cells intact.Results. Overall, HNPCs survived well for up to six weeks in all groups. However, transplants receiving the BDNF-containing PA gel demonstrated significantly higher numbers of HNPCs and neuronal differentiation. At six weeks, a majority of the HNPCs had migrated into the brain stem and differentiated. Differentiated human cells as well as neurites were observed in the vicinity of the cochlear nucleus.Conclusion. Our results indicate that human neural precursor cells (HNPC) integration with host tissue benefits from additional brain derived neurotrophic factor (BDNF) treatment and that these cells appear to be good candidates for further regenerative studies on the auditory nerve (AN). |
| Databáze: | OpenAIRE |
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