Vitrification of mouse oocytes in ethylene glycol-raffinose solution: effects of preexposure to ethylene glycol or raffinose on oocyte viability
| Autor: | Seiji Katagiri, Edwin C. Atabay, Masashi Nagano, Yoshiyuki Takahashi, E.C. dela Peña |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Ethylene Glycol
Cell Survival Volume change In Vitro Techniques General Biochemistry Genetics and Molecular Biology Cryopreservation Andrology chemistry.chemical_compound Mice Cryoprotective Agents Raffinose medicine Animals Vitrification Dehydration Metaphase ii General Medicine Oocyte medicine.disease Mice Inbred C57BL Solutions medicine.anatomical_structure chemistry Biochemistry Mice Inbred CBA Oocytes Female General Agricultural and Biological Sciences Ethylene glycol |
| Zdroj: | Cryobiology. 42(2) |
| ISSN: | 0011-2240 |
| Popis: | We investigated the effects of preexposure to ethylene glycol (EG) or raffinose on the viability of vitrified mouse oocytes. Ovulated oocytes at the metaphase II stage were preexposed either to 2 M EG for 0, 2, or 5 min or to ascending concentrations (0.15 followed by 0.3 M ) of raffinose solution for 2, 5, or 10 min each (here referred to as 2-2, 5-5, and 10-10 min, respectively). The oocytes were then exposed to a vitrification solution (VS), 6 M EG + 0.3 M raffinose, for 0.5, 1, 2, or 5 min and then vitrified or immediately diluted. After warming, the developmental capacity of oocytes was determined after in vitro fertilization. Volume changes in oocytes during preexposures and exposure to the VS were also investigated. The results demonstrated that preexposure to 2 M EG allowed shorter exposure times of oocytes to the VS and that predehydration in raffinose solutions for 5-5, but not 2-2 or 10-10 min, allowed a wider range of exposure times to the VS. Experiments on volume change suggested that the optimum time of exposure to the VS depends on the amount of EG permeation after preexposure to 2 M EG or to raffinose solutions. Preexposures to 2 M EG or raffinose under optimized conditions increased the viability of vitrified-warmed oocytes compared to direct exposure to VS without preexposures. |
| Databáze: | OpenAIRE |
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