Deletion of the serotonin transporter in rats disturbs serotonin homeostasis without impairing liver regeneration
| Autor: | Carine Punt, Ronald Kisjes, M.J.M. Toussaint, Alain de Bruin, Ramadhan B. Matondo, Judith R. Homberg, Suzanne J.A. Korporaal, Edwin Cuppen, Jan Willem N. Akkerman |
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| Přispěvatelé: | Hubrecht Institute for Developmental Biology and Stem Cell Research |
| Rok vydání: | 2009 |
| Předmět: |
Blood Platelets
medicine.medical_specialty Serotonin Knockout rat Bleeding Time Physiology chemistry.chemical_compound Immune Regulation [NCMLS 2] Translational research [ONCOL 3] Physiology (medical) Internal medicine medicine Animals Hepatectomy Homeostasis Platelet Neurotransmitter Serotonin transporter Serotonin Plasma Membrane Transport Proteins Hereditary cancer and cancer-related syndromes [ONCOL 1] Hepatology biology Gastroenterology Transporter Liver regeneration Liver Regeneration Rats Endocrinology chemistry biology.protein Functional Neurogenomics [DCN 2] Gene Deletion |
| Zdroj: | American Journal of Physiology : Gastrointestinal and Liver Physiology, 296, G963-8 American Journal of Physiology : Gastrointestinal and Liver Physiology, 296, 4, pp. G963-8 American Journal of Physiology-Gastrointestinal and Liver Physiology, 296(4), 963-968. American Physiological Society |
| ISSN: | 0193-1857 |
| Popis: | Item does not contain fulltext The serotonin transporter is implicated in the uptake of the vasoconstrictor serotonin from the circulation into the platelets, where 95% of all blood serotonin is stored and released in response to vascular injury. In vivo studies indicated that platelet-derived serotonin mediates liver regeneration after partial hepatectomy. We have recently generated serotonin transporter knockout rats and demonstrated that their platelets were almost completely depleted of serotonin. Here we show that these rats exhibit impaired hemostasis and contain about 1-6% of wild-type serotonin levels in the blood. Despite the marked reduction of serotonin levels in blood and platelets, efficient liver regeneration and collagen-induced platelet aggregation occur in rats lacking the serotonin transporter. These results provide evidence that liver regeneration is not dependent on the release of serotonin from platelets. Our findings indicate that very low levels of serotonin in blood are sufficient for liver regeneration. |
| Databáze: | OpenAIRE |
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