Effects of psoralen plus UVA radiation (PUVA) on HIV-1 in human beings: A pilot study
| Autor: | Thomas Horn, Barbara Z. Zmudzka, Warwick L. Morison, Homayoon Farzadegan, Janusz Z. Beer |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
CD4-Positive T-Lymphocytes
UVA Radiation HIV Core Protein p24 Human immunodeficiency virus (HIV) Pilot Projects Genome Viral Dermatology medicine.disease_cause Skin Diseases Peripheral blood mononuclear cell law.invention Flow cytometry chemistry.chemical_compound law UV Radiation Exposure medicine Humans PUVA Therapy Polymerase chain reaction Psoralen AIDS-Related Opportunistic Infections medicine.diagnostic_test business.industry Peripheral blood chemistry DNA Viral Immunology HIV-1 RNA Viral Virus Activation business |
| Zdroj: | Journal of the American Academy of Dermatology. 31:735-740 |
| ISSN: | 0190-9622 |
| DOI: | 10.1016/s0190-9622(94)70234-9 |
| Popis: | Background: Laboratory data document the activation of the HIV-1 genome on exposure to UV radiation, including PUVA. The overall effects of UV radiation exposure on HIV-1 infection in human beings are unknown. Objective: Our purpose was to observe CD4 cell counts and quantitative markers of HIV-1 load in late-stage HIV-1-infected human beings receiving PUVA for various cutaneous diseases. Methods: Samples of peripheral blood were obtained on days 0, 14, 30, and 60 of PUVA administered in therapeutic doses. Number of CD4+ T lymphocytes was determined by flow cytometry, and HIV-1 load was measured by semiquantitative polymerase chain reaction for viral genome in peripheral blood mononuclear cells, semiquantitative RNA-polymerase chain reaction for HIV-1 RNA in serum, and determination of p24 in serum. Results: No significant changes in the measurements were observed. Conclusion: This study did not detect a deleterious effect on CD4 cell count or HIV-1 load during 2 months of PUVA treatment for patients in late stages of infection, with low CD4 cell counts and high HIV-1 loads. |
| Databáze: | OpenAIRE |
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