Inheritance of physico-chemical properties and ROS generation by carbon quantum dots derived from pyrolytically carbonized bacterial sources
| Autor: | Yanyan Wu, Hao Wei, H. C. van der Mei, J. de Vries, Henk J. Busscher, Yijin Ren |
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| Přispěvatelé: | Personalized Healthcare Technology (PHT), Man, Biomaterials and Microbes (MBM) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Medicine (General)
X-ray photoelectron spectroscopy QH301-705.5 Probiotic bacteria Biomedical Engineering Infrared spectroscopy chemistry.chemical_element Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Bacterial cell structure Biomaterials chemistry.chemical_compound R5-920 Full Length Article Amide Biology (General) Molecular Biology Chemical composition 2. Zero hunger biology Chemistry Carbonization technology industry and agriculture Infrared-spectroscopy Cell Biology 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences 3. Good health Chemical engineering Pathogenic bacteria Degradation (geology) 0210 nano-technology Reactive oxygen species Carbon Bacteria Biotechnology |
| Zdroj: | Materials Today Bio Materials Today Bio, 12:100151. Elsevier Materials Today Bio, Vol 12, Iss, Pp 100151-(2021) |
| ISSN: | 2590-0064 |
| Popis: | Bacteria are frequently used in industrial processes and nutrient supplementation to restore a healthy human microflora, but use of live bacteria is often troublesome. Here, we hypothesize that bacterially-derived carbon-quantum-dots obtained through pyrolytic carbonization inherit physico-chemical properties from probiotic and pathogenic source-bacteria. Carbon-quantum-dots carbonized at reaction-temperatures below 200 °C had negligible quantum-yields, while temperatures above 220 °C yielded poor water-suspendability. Fourier-transform infrared-spectroscopy demonstrated preservation of amide absorption bands in carbon-quantum-dots derived at intermediate temperatures. X-ray photoelectron-spectroscopy indicated that the at%N in carbon-quantum-dots increased with increasing amounts of protein in source-bacterial surfaces. Carbonization transformed hydrocarbon-like bacterial surface compounds into heterocyclic aromatic-carbon structures, evidenced by a broad infrared absorption band (920-900 cm−1) and the presence of carbon in C–C functionalities of carbon-quantum-dots. The chemical composition of bacterially-derived carbon-quantum-dots could be explained by the degradation temperatures of main bacterial cell surface compounds. All carbon-quantum-dots generated reactive-oxygen-species, most notably those derived from probiotic lactobacilli, carrying a high amount of surface protein. Concluding, amide functionalities in carbon-quantum-dots are inherited from surface proteins of source-bacteria, controlling reactive-oxygen-species generation. This paves the way for applications of bacterially-derived carbon-quantum-dots in which reactive-oxygen-species generation is essential, instead of hard-to-use live bacteria, such as in food supplementation or probiotic-assisted antibiotic therapy. Graphical abstract Image 1 Highlights • Pyrolytic carbonization of bacteria between 200°C and 220°C yields water-suspendable CQDs. • Bacterially-derived CQDs inherit amide functionalities from bacterial cell surface proteins. • Hydrocarbon-like bacterial surface compounds give heterocyclic aromatic-carbon structures in bacterially-derived CQDs. • Bacterially-derived CQDs possess graphitic nitrogen. • Zeta potentials of CQDs relate with nitrogen occurrence in CQDs. |
| Databáze: | OpenAIRE |
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