Chronic administration of methionine and/or methionine sulfoxide alters oxidative stress parameters and ALA-D activity in liver and kidney of young rats

Autor: Jucimara Baldissarelli, Carla Roberta Nunes Polachini, Pathise Souto Oliveira, Maria Rosa Chitolina Schetinger, Gabriela Debom, Roselia Maria Spanevello, Rejane Giacomelli Tavares, Mayara Sandrielly Pereira Soares, Francieli Moro Stefanello, Vera Maria Morsch, Bruna da Silveira de Mattos
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Clinical Biochemistry
Glycine N-Methyltransferase
medicine.disease_cause
Kidney
Biochemistry
Lipid peroxidation
chemistry.chemical_compound
Methionine
Urea
chemistry.chemical_classification
biology
Glutathione peroxidase
Catalase
Cholesterol
Liver
Female
Hypermethioninemia
medicine.medical_specialty
Injections
Subcutaneous
Thiobarbituric Acid Reactive Substances
Superoxide dismutase
03 medical and health sciences
Internal medicine
medicine
TBARS
Animals
Sulfhydryl Compounds
Rats
Wistar
Amino Acid Metabolism
Inborn Errors
Triglycerides
Glutathione Peroxidase
Superoxide Dismutase
Organic Chemistry
Porphobilinogen Synthase
medicine.disease
Rats
Enzyme Activation
Oxidative Stress
030104 developmental biology
Endocrinology
Glucose
chemistry
biology.protein
Lipid Peroxidation
Reactive Oxygen Species
Oxidative stress
Zdroj: Amino acids. 49(1)
ISSN: 1438-2199
Popis: High levels of methionine (Met) and methionine sulfoxide (MetO) are found in several genetic abnormalities. Oxidative stress is involved in the pathophysiology of many inborn errors of metabolism. However, little is known about the role of oxidative damage in hepatic and renal changes in hypermethioninemia. We investigated the effect of chronic treatment with Met and/or MetO on oxidative stress parameters in liver and kidney, as lipid peroxidation (TBARS), total sulfhydryl content (SH), reactive oxygen species (ROS) and enzymes activities superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and delta aminolevulinic dehydratase (ALA-D). Serum biochemical parameters were evaluated. Wistar rats were treated daily with two subcutaneous injections of saline (control), Met (0.2–0.4 g/kg), MetO (0.05–0.1 g/kg) and the association between these (Met plus MetO) from the 6th to the 28th day of life. Our data demonstrated an increase of glucose and urea levels in all experimental groups. Cholesterol (MetO and Met plus MetO) were decreased and triglycerides (MetO) were increased. SOD (MetO and Met plus MetO) and CAT (Met, MetO and Met plus MetO) activities were decreased, while GPx was enhanced by MetO and Met plus MetO treatment in liver. In kidney, we observed a reduction of SH levels, SOD and CAT activities and an increase of TBARS levels in all experimental groups. ROS levels in kidney were increased in MetO and Met plus MetO groups. ALA-D activity was enhanced in liver (MetO and Met plus MetO) and kidney (Met plus MetO). These findings help to understand the pathophysiology of hepatic and renal alterations present in hypermethioninemia.
Databáze: OpenAIRE
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