Interferometric porous silicon transducers using an enzymatically amplified optical signal
| Autor: | Endre J. Szili, Martin J. Sweetman, Suet P. Low, Nicolas H. Voelcker, Andrew Jane, Roger St. C. Smart, Sunil Kumar, Peter J. Macardle |
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| Přispěvatelé: | Szili, Endre J, Jane, Andrew, Low, Suet P, Sweetman, Martin, Macardle, Peter, Kumar, Sunil, Smart, Roger St C, Voelcker, Nicolas H |
| Rok vydání: | 2011 |
| Předmět: |
Materials science
3 3' 5 5' -Tetramethylbenzidine Analytical chemistry Porous silicon Signal Horseradish peroxidase chemistry.chemical_compound Materials Chemistry Electrical and Electronic Engineering Instrumentation biology horseradish peroxidase business.industry technology industry and agriculture Metals and Alloys interferometric reflectance spectroscopy 3 3' 5 5'-Tetramethylbenzidine equipment and supplies Condensed Matter Physics human immunoglobulin Surfaces Coatings and Films Electronic Optical and Magnetic Materials Interferometry porous silicon Transducer chemistry biology.protein Optoelectronics business Biosensor Layer (electronics) |
| Zdroj: | Sensors and Actuators B: Chemical. 160:341-348 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2011.07.059 |
| Popis: | This paper describes a novel transducer principle converting biomolecular binding events into changes in porous silicon structural colour. An enzyme-catalysed reaction, horseradish peroxidase (HRP) mediated oxidation of 3,3 ,5,5 -tetramethylbenzidine (TMB), induced an amplified optical detection signal on an antibody-functionalised porous silicon film, which manifested as significant changes in the Fabry–Pérot fringe pattern. Exposing porous silicon to non-oxidised TMB and oxidised TMB products and measuring the resulting effective optical thickness (EOT) changes of the porous silicon layer, an amplified signal from porous silicon was found, caused by an intermediate radical cation produced during the enzymaticcatalysed oxidation of TMB. Finally, it was shown that this system could be used to detect a human immunoglobulin (IgG) at 0.2 g/ml. This work may lead to the development of new biosensors where porous silicon acts as the sensing matrix and transducer element. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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