MicroRNA-204 may participate in the pathogenesis of hypoxic-ischemic encephalopathy through targeting KLLN
| Autor: | Ronglin Chen, Jiefu Lu, Feng Cao, Shaopin Fu, Pengkai Duan, Meixia Wang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research killin p53 regulated DNA replication inhibitor Pathogenesis 03 medical and health sciences 0302 clinical medicine Immunology and Microbiology (miscellaneous) Western blot Medicine hypoxic-ischemic encephalopathy Reporter gene Oncogene medicine.diagnostic_test business.industry pathogenesis General Medicine Transfection Articles Cell cycle 030104 developmental biology Terminal deoxynucleotidyl transferase Apoptosis 030220 oncology & carcinogenesis Cancer research microRNA-204 business |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-0981 |
| Popis: | Hypoxic-ischemic encephalopathy (HIE) is a common neonatal disease that can lead to high neonatal mortality rates. Previous studies have indicated that microRNAs (miRs) may be involved in the pathogenesis of HIE; however, the specific mechanisms underlying their involvement require further investigation. The aim of the present study was to investigate the roles of miR-204 and its target gene killin p53 regulated DNA replication inhibitor (KLLN) in HIE using rat HIE models. Brain injury was induced by surgery and incubation of hypoxic incubator brain using 10-day-old pup rats. On day 3, rats were sacrificed, and the infarct size of the brain was determined using a tetrazolium chloride assay. Terminal deoxynucleotidyl transferase UTP nick-end labeling staining was performed to detect the cell death rate in the brain tissue. Following this, the brain tissues were collected, and reverse transcription-quantitative polymerase chain reaction, western blot analysis and immunohistochemistry assays were performed to examine the expression levels of miR-204 and KLLN. Furthermore, neurons were cultured and transfected with miR-204 inhibitors or mimics, and the effect of miR-204 on the proliferation and apoptosis of neurons was examined using MTT and flow cytometric assays. Finally, a dual-luciferase reporter assay was performed to confirm whether KLLN is a direct target of miR-204. The expression of miR-204 was significantly downregulated and the expression of KLLN was significantly increased in the brain tissue of HIE rats (P |
| Databáze: | OpenAIRE |
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