Green synthesis of silver nanoparticles by Rivina humilis leaf extract to tackle growth of Brucella species and other perilous pathogens
| Autor: | Sri Raghava, Sharanaiah Umesha, Kenneth Munnene Mbae |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hemagglutination Biocompatibility Characterization Dispersity Nanoparticle 01 natural sciences Silver nanoparticle Brucellosis 03 medical and health sciences lcsh:QH301-705.5 biology Chemistry Rivina humilis Antimicrobial Haemolysis biology.organism_classification 030104 developmental biology lcsh:Biology (General) Original Article Silver nanoparticles General Agricultural and Biological Sciences 010606 plant biology & botany Nuclear chemistry |
| Zdroj: | Saudi Journal of Biological Sciences Saudi Journal of Biological Sciences, Vol 28, Iss 1, Pp 495-503 (2021) |
| ISSN: | 2213-7106 1319-562X |
| Popis: | Novel approaches are obligatory to treat chronic intracellular bacterial infectious diseases like Brucellosis specifically, are very complicated to deal with. The aim of the study to take upon nanotechnology approach to exploit the efficacy of the synthesized nanoparticles, to overcome barriers for treatment of Brucella species and other pathogens. Present study used Rivina humilis extract as reductant of silver ions for synthesis of silver nanoparticles for the first time. Rh-AgNP’s was characterized by UV–visible spectroscopy, DLS, FT-IR, SEM, EDS, TEM and XRD. Radical scavenging, antibrucellosis, bactericidal activity was evaluated. Clinical application was assessed by Rate of haemolysis, fibrinolytic and Hemagglutination activity. UV–visible spectrum of synthesized Rh-AgNP’s showed maximum peak at 440 nm indicating the formation of nanoparticles. TEM showed that the average particle size of nanoparticles 51 nm with spherical shape, DLS depicted monodisperse state in water; EDS confirmed the presence of silver metal. Rh-AgNP’s exhibited potential antibrucellosis activity against B. abortus, B. melitensis and B. suis effective inhibition at 800 μg/mL. The bio-compatibility of Rh-AgNP’s was established by rate of haemolysis, hemagglutination and fibrinolytic activity. For the first time it has been proved that Rh-AgNP’s have efficacy as antimicrobial agent with potential application in the biological domain. |
| Databáze: | OpenAIRE |
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