Effects of straight alkyl chain, extra hydroxylated alkyl chain and branched chain amino acids on gastric emptying evaluated using a non-invasive breath test in conscious rats
| Autor: | Kimiko Shimizu, Kaori Iwasawa, Orie Kobayashi, Masayuki Uchida |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
Threonine Consciousness Physiology Original Cmax Glycine Administration Oral Gastric emptying Rats Sprague-Dawley 03 medical and health sciences Acetic acid chemistry.chemical_compound 0302 clinical medicine Leucine medicine Serine Animals Amino Acids Isoleucine Breath test in rats Alkyl Acetic Acid Breath test chemistry.chemical_classification Chromatography Alanine medicine.diagnostic_test Non invasive digestive oral and skin physiology Valine General Medicine Amino acid chemistry Distilled water Breath Tests 030211 gastroenterology & hepatology 030217 neurology & neurosurgery Amino Acids Branched-Chain |
| Zdroj: | Journal of Smooth Muscle Research |
| ISSN: | 1884-8796 0916-8737 |
| Popis: | AIM Some amino acids been known to influence gastric emptying. Thus we have evaluated the effects of straight alkyl chain, extra hydroxylated alkyl chain and branched chain amino acids on gastric emptying. MATERIALS AND METHODS Gastric emptying was evaluated in rats after feeding with Racol (nutrient formulae) containing [1-(13)C] acetic acid. Using a breath test, the content of (13)CO2 in their expired air was measured by infrared analyzers. Rats were orally administered with test amino acids, while control rats were administered orally with distilled water. RESULTS The expired (13)CO2 content in the expired air increased with time, peaked after about 30 min and decreased thereafter. Among the amino acids having an alkyl chain, L-serine, L-alanine and L-glycine, significantly decreased the (13)CO2 content and Cmax, and delayed Tmax, suggesting inhibition and delay of gastric emptying. AUC(120min) values of L-alanine and L-glycine also decreased significantly. L-Threonine significantly decreased (13)CO2 content and delayed Tmax, but had no influence on Cmax and AUC(120min) values, suggesting a delay of gastric emptying. L-Isoleucine and L-leucine and L-valine significantly decreased (13)CO2 content, suggesting inhibition of the gastric emptying, but Cmax, Tmax and AUC(120min) values were not significantly affected. CONCLUSION The results show that the amino acids used in the present study had different effects on gastric emptying. Moreover, it was found that inhibition and delay of gastric emptying were clearly classifiable by analyzing the change in (13)CO2 content of the expired air and the Cmax, Tmax and AUC(120min) values. |
| Databáze: | OpenAIRE |
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