Arabidopsis AtVPS15 Plays Essential Roles in Pollen Germination Possibly by Interacting with AtVPS34
| Autor: | Genji Qin, Wei-Ying Wang, Zhiqiang Ma, Shufan Xing, Hongya Gu, Li Zhang, Li-Jia Qu, Jingjing Liu |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Mutant
Arabidopsis Germination GUS reporter system Real-Time Polymerase Chain Reaction medicine.disease_cause Vacuolar Sorting Protein VPS15 chemistry.chemical_compound Gene Expression Regulation Plant Two-Hybrid System Techniques Pollen Botany Genetics medicine DAPI Molecular Biology Gametophyte biology Arabidopsis Proteins food and beverages Plants Genetically Modified biology.organism_classification Class III Phosphatidylinositol 3-Kinases Yeast Cell biology chemistry Protein Binding |
| Zdroj: | Journal of Genetics and Genomics. 39:81-92 |
| ISSN: | 1673-8527 |
| Popis: | VPS15 protein is a component of the phosphatidylinositol 3-kinase complex which plays a pivotal role in the development of yeast and mammalian cells. The knowledge about the function of its homologue in plants remains limited. Here we report that AtVPS15, a homologue of yeast VPS15p in Arabidopsis, plays an essential role in pollen germination. Homozygous T-DNA insertion mutants of AtVPS15 could not be obtained from the progenies of self-pollinated heterozygous mutants. Reciprocal crosses between atvps15 mutants and wild-type Arabidopsis revealed that the T-DNA insertion was not able to be transmitted by male gametophytes. DAPI staining, Alexander's stain and scanning electron microscopic analysis showed that atvps15 heterozygous plants produced pollen grains that were morphologically indistinguishable from wild-type pollen, whereas in vitro germination experiments revealed that germination of the pollen grains was defective. GUS staining analysis of transgenic plants expressing the GUS reporter gene driven by the AtVPS15 promoter showed that AtVPS15 was mainly expressed in pollen grains. Finally, DUALmembrane yeast two-hybrid analysis demonstrated that AtVPS15 might interact directly with AtVPS34. These results suggest that AtVPS15 is very important for pollen germination, possibly through modulation of the activity of PI3-kinase. |
| Databáze: | OpenAIRE |
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