Fluorescence in situ hybridization of three oncogenes on a leiomyoma
| Autor: | Mohammad Saquib, Joel S. Noumoff, Shamim A. Faruqi, Christopher Harsch |
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| Rok vydání: | 2012 |
| Předmět: |
Transcriptional Activation
Histology Receptors Retinoic Acid Cyclin D Gene Dosage Genes myc DNA Satellite Polyploid Cells Proto-Oncogene Proteins c-myc medicine Humans Instrumentation Gene In Situ Hybridization Fluorescence Cyclin Uterine leiomyoma Leiomyoma medicine.diagnostic_test biology Chromosomes Human Pair 11 Retinoic Acid Receptor alpha medicine.disease Molecular biology Medical Laboratory Technology biology.protein Cancer gene Female Anatomy Fluorescence in situ hybridization |
| Zdroj: | Microscopy Research and Technique. 75:1147-1149 |
| ISSN: | 1059-910X |
| Popis: | Using fluorescence in situ hybridization technique, expression of three oncogenes, C-myc, RARa, and cyclin-D was tested on a uterine leiomyoma. C-myc and RARa were amplified in approximately 30% and 90% of the cells, respectively. Numerous small signals of C-myc were indicative of the presence of double minutes. Amplification of RARa is being reported for the first time in a leiomyoma. Cyclin-D was normal in diploid cells while it was highly amplified in polyploid cells. Low levels of amplified C-myc and cyclin-D cells seem to be the reason for this tumor to be benign, while RARa could not be effective without the association of some other gene such as PML. Information presented here are significant toward developing new curative strategies such as gene-specific drugs and molecular manipulation to stop the activity of cancer gene. Further study may elucidate that how fibroids grow and maintain their rare benign nature. Microsc. Res. Tech. 75:1147–1149, 2012. © 2012 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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