A New SARS-CoV-2 Dual-Purpose Serology Test: Highly Accurate Infection Tracing and Neutralizing Antibody Response Detection
| Autor: | Sean C. Taylor, Kyle Annen, Beth Hurst, Vijaya Knight, Thomas E. Morrison, Melkon G DomBourian, Ashley Bailey, Jamil N. Kanji, Carmen L. Charlton, Leah Huey, Mary K. McCarthy |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Dual purpose viruses Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serology Antibodies Viral Neutralization Serology Immunity Virology Humans Medicine neutralizing antibodies Neutralizing antibody biology SARS-CoV-2 business.industry COVID-19 Spike Protein vaccines Antibodies Neutralizing Spike Glycoprotein Coronavirus biology.protein ELISA Antibody business |
| Zdroj: | Journal of Clinical Microbiology |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Many severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serology tests have proven to be less accurate than expected and do not assess antibody function as neutralizing, correlating with protection from reinfection. A new assay technology measuring the interaction of the purified SARS-CoV-2 spike protein receptor binding domain (RBD) with the extracellular domain of the human angiotensin-converting enzyme 2 (hACE2) receptor detects these important antibodies. Many severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serology tests have proven to be less accurate than expected and do not assess antibody function as neutralizing, correlating with protection from reinfection. A new assay technology measuring the interaction of the purified SARS-CoV-2 spike protein receptor binding domain (RBD) with the extracellular domain of the human angiotensin-converting enzyme 2 (hACE2) receptor detects these important antibodies. The cPass surrogate virus neutralization test (sVNT), compared directly with eight SARS-CoV-2 IgG serology and two live-cell neutralization tests, gives similar or improved accuracy for qualitative delineation between positive and negative individuals in a fast, scalable, and high-throughput assay. The combined data support the cPass sVNT as a tool for highly accurate SARS-CoV-2 immunity surveillance of infected/recovered and/or vaccinated individuals as well as drug and convalescent-phase donor screening. The data also preview a novel application for the cPass sVNT in calibrating the stringency of live-cell neutralization tests and its use in longitudinal testing of recovered and/or vaccinated patients. |
| Databáze: | OpenAIRE |
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