Evidence for Extrathymic Generation of Regulatory T Cells Specific for a Retinal Antigen
| Autor: | Dale S. Gregerson, Scott W. McPherson, Neal D. Heuss, Ute Lehmann |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adoptive cell transfer
Apoptosis Enzyme-Linked Immunosorbent Assay Mice Transgenic Thymus Gland Biology Lymphocyte Activation Autoantigens T-Lymphocytes Regulatory Retina Photoreceptor cell Autoimmune Diseases Immune tolerance Mice Cellular and Molecular Neuroscience Antigen Immune Tolerance medicine Animals Cytotoxic T cell IL-2 receptor Antigen-presenting cell Cells Cultured Retinitis Peripheral tolerance Uveitis Posterior General Medicine beta-Galactosidase Adoptive Transfer eye diseases Sensory Systems Cell biology Disease Models Animal Ophthalmology medicine.anatomical_structure Immunology sense organs Photoreceptor Cells Vertebrate |
| Zdroj: | Ophthalmic Research. 40:154-159 |
| ISSN: | 1423-0259 0030-3747 |
| DOI: | 10.1159/000119868 |
| Popis: | Background: Thymic expression of a photoreceptor cell antigen, interphotoreceptor retinoid-binding protein, is known to generate regulatory T cells (Treg) that prevent spontaneous autoimmune disease of the retina. However, the contribution of other endogenous, tissue-specific antigens (Ags) expressed in the retina to the generation of Treg is uncertain. Methods: Transgenic mice that express β-galactosidase (β-gal) in photoreceptor cells, together with β-gal-specific T cell receptor transgenic mice, were used to study the induction of Treg in vivo. Results: Transgenic expression of β-gal on the arrestin promoter led to a spontaneous immunoregulatory response that inhibited the development of immune responses to β-gal. The regulation was transferred by CD3+4+25+ Treg. Several strategies were then used to show that β-gal expressed in the retina supported spontaneous, thymus-independent Treg development. The endogenous Treg also differed from the Treg induced by Ag inoculation into the anterior chamber of the eye. Conclusion: These results demonstrate that retinal expression of very small amounts of a tissue-specific Ag can generate Treg in the periphery. |
| Databáze: | OpenAIRE |
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