p53, c-erbB-2 and nm23 expression have no prognostic significance in primary pulmonary adenocarcinoma
| Autor: | Cockburn Js, Robert R. Jeffrey, Kerr Km, G King, MacKinnon M, Kennedy Mm |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Lung Neoplasms Receptor ErbB-2 medicine.drug_class Adenocarcinoma Monoclonal antibody chemistry.chemical_compound Biomarkers Tumor medicine Humans Stage (cooking) Lung Monomeric GTP-Binding Proteins biology business.industry Respiratory disease General Medicine Middle Aged NM23 Nucleoside Diphosphate Kinases Prognosis medicine.disease medicine.anatomical_structure Antigen retrieval chemistry Nucleoside-Diphosphate Kinase biology.protein Immunohistochemistry Female Surgery Tumor Suppressor Protein p53 Antibody Cardiology and Cardiovascular Medicine business Transcription Factors |
| Zdroj: | European Journal of Cardio-Thoracic Surgery. 11:838-842 |
| ISSN: | 1010-7940 |
| DOI: | 10.1016/s1010-7940(97)01162-7 |
| Popis: | Objective: We analysed nm23, c-erbB-2 and p53 protein expression in lung adenocarcinoma in relation to clinicopathological status and patient survival, to elucidate any potential prognostic value. Published reports suggest that high p53 and c-erbB-2 protein expression and loss of nm23 protein expression are associated with poor prognosis. Methods: A total of 162 pulmonary adenocarcinomas resected between 1980 and 1991 were stained using monoclonal antibodies to nm23 (NCL-nm23), c-erbB-2 (NCL-CB11) and p53 (DAKO Do7). Antigen retrieval was by microwave heating and bound antibody was visualised using standard immunohistochemical methods. Staining was scored by two observers blinded to tumour status and patient survival. Results: Of the tumours, 101/162 (62.3%) exhibited high-level p53 expression, 30 (18.5%) showed high-level c-erbB-2 membrane staining, and 77 (47.5%) demonstrated loss of nm23 positivity. The influence of T and N status and disease stage on postoperative survival was as expected. The predicted effect on patient survival of nm23, c-erbB-2 or nm23 protein expression suggested by previous studies was not verified by our work. This was true both overall and for groups within the same T or N status or stage. Conclusion: Immunohistochemical assessment of the nm23, c-erbB-2 and p53 proteins using the above monoclonal antibodies does not have value as an independent prognostic indicator in pulmonary adenocarcinoma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|