Profiling locomotor recovery: comprehensive quantification of impairments after CNS damage in rodents
| Autor: | Roman Gonzenbach, Hansjörg Kasper, Martina Röthlisberger, Linard Filli, Michelle L. Starkey, Björn Zörner, Marc Bolliger, Martin E. Schwab |
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| Přispěvatelé: | University of Zurich, Zörner, B |
| Rok vydání: | 2010 |
| Předmět: |
Central Nervous System
1303 Biochemistry Central nervous system 610 Medicine & health Walking Biochemistry 1307 Cell Biology Mice 1312 Molecular Biology medicine Animals Gait Molecular Biology Swimming Analysis method 10242 Brain Research Institute business.industry Overground walking Cell Biology Spinal cord Rats Mice Inbred C57BL medicine.anatomical_structure Science research Rats Inbred Lew 1305 Biotechnology 570 Life sciences biology 10046 Balgrist University Hospital Swiss Spinal Cord Injury Center Female Psychomotor Disorders Psychomotor disorder business Neuroscience Locomotion Psychomotor Performance Biotechnology |
| Zdroj: | Nature Methods. 7:701-708 |
| ISSN: | 1548-7105 1548-7091 |
| DOI: | 10.1038/nmeth.1484 |
| Popis: | Rodents are frequently used to model damage and diseases of the central nervous system (CNS) that lead to functional deficits. Impaired locomotor function is currently evaluated by using scoring systems or biomechanical measures. These methods often suffer from limitations such as subjectivity, nonlinearity and low sensitivity, or focus on a few very restricted aspects of movement. Thus, full quantitative profiles of motor deficits after CNS damage are lacking. Here we report the detailed characterization of locomotor impairments after applying common forms of CNS damage in rodents. We obtained many objective and quantitative readouts from rats with either spinal cord injuries or strokes and from transgenic mice (Epha4−/−) during skilled walking, overground walking, wading and swimming, resulting in model-specific locomotor profiles. Our testing and analysis method enables comprehensive assessment of locomotor function in rodents and has broad application in various fields of life science research. |
| Databáze: | OpenAIRE |
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