The Promotion of Erythropoiesis via the Regulation of Reactive Oxygen Species by Lactic Acid
| Autor: | Shun-Tao Luo, Lian Lu, Dong-Mei Zhang, Min Luo, Li Jiang, Qing Qin, Yuquan Wei, Bin Shao, Huashan Shi, Hanshuo Yang, Fuchun Guo, Meng Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gene Expression Article 03 medical and health sciences chemistry.chemical_compound Hemoglobins Bone Marrow hemic and lymphatic diseases medicine Animals Humans Erythropoiesis Lactic Acid chemistry.chemical_classification Erythroid Precursor Cells Reactive oxygen species Mice Inbred BALB C Multidisciplinary Chemistry Cell Differentiation Hematopoietic Stem Cells In vitro Lactic acid Haematopoiesis 030104 developmental biology medicine.anatomical_structure Biochemistry Female Bone marrow Stem cell K562 Cells Reactive Oxygen Species K562 cells |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep38105 |
| Popis: | The simultaneous increases in blood lactic acid and erythrocytes after intense exercise could suggest a link between lactate and the erythropoiesis. However, the effects of lactic acid on erythropoiesis remain to be elucidated. Here, we utilized a mouse model to determine the role of lactic acid in this process in parallel with studies using leukaemic K562 cells. Treatment of K562 cells in vitro with lactic acid increased the mRNA and protein expression of haemoglobin genes and the frequency of GPA+ cells. Also, increases in haematocrit and CD71−/Ter119+ erythroid cells were observed in lactic acid-treated mice, which showed a physiological increase in blood lactate. Mouse bone marrow CD34+/CD117− cells showed an increase in erythroid burst-forming units after stimulation with lactic acid in vitro. Furthermore, lactic acid increased the intracellular reactive oxygen species (ROS) content in bone marrow and in K562 cells. Erythroid differentiation induced in Haematopoietic Stem Cells (HSCs) and K562 cells by lactic acid was abolished by reducing ROS levels with SOD or 2-mercaptoethanol, which suggests that ROS is a critical regulator of this process. These findings provide a better understanding of the role of lactic acid in cellular metabolism and physiological functions. |
| Databáze: | OpenAIRE |
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