The Adaptive Response to Dietary Zinc in Mice Involves the Differential Cellular Localization and Zinc Regulation of the Zinc Transporters ZIP4 and ZIP5
| Autor: | Glen K. Andrews, Yien-Ming Kuo, Jodi Dufner-Beattie, Jane Gitschier |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
DNA Complementary Time Factors Blotting Western Molecular Sequence Data chemistry.chemical_element Zinc Biology Biochemistry Evolution Molecular Mice Internal medicine Gene expression medicine Animals Insulin Tissue Distribution Secretion Amino Acid Sequence Yolk sac Cation Transport Proteins Pancreas Molecular Biology Conserved Sequence Cellular localization Yolk Sac Models Genetic Sequence Homology Amino Acid Cell Membrane Endoderm Acrodermatitis enteropathica Computational Biology Gene Expression Regulation Developmental Biological Transport Transporter Exons Cell Biology Blotting Northern medicine.disease Immunohistochemistry Endocrinology medicine.anatomical_structure Microscopy Fluorescence chemistry Dietary Supplements Zinc deficiency RNA Female |
| Zdroj: | Journal of Biological Chemistry. 279:49082-49090 |
| ISSN: | 0021-9258 |
| Popis: | The ZIP5 gene encodes a protein closely related to ZIP4, a zinc transporter mutated in the human genetic disorder acrodermatitis enteropathica. Herein, we demonstrate that mouse ZIP5 and ZIP4 genes are co-expressed in several tissues involved in zinc homeostasis (intestine, pancreas, embryonic yolk sac). However, unlike expression of the ZIP4 gene, which is induced during periods of zinc deficiency, ZIP5 gene expression is unaltered by dietary zinc. Immunohistochemistry localizes ZIP5 to the basolateral surfaces of enterocytes, acinar cells, and visceral endoderm cells in mice fed a zinc-adequate diet. However, this protein is removed from these cell surfaces and internalized during dietary zinc deficiency. In contrast, ZIP4 is induced and recruited to the apical surface of enterocytes and endoderm cells during zinc deficiency. In the pancreas, ZIP4 is expressed in beta-cells, whereas ZIP5 is expressed in acinar cells. These results suggest that the function of ZIP5 is antagonistic to that of ZIP4 in the control of zinc homeostasis; rather than functioning in the acquisition of dietary zinc, as does ZIP4, ZIP5 may function in the removal of zinc from the body. Thus, during periods when dietary zinc is replete, ZIP5 may function to remove zinc from the blood via the pancreas and intestine, the major sites of zinc excretion in mammals, whereas the acquisition of dietary zinc by intestinal ZIP4 would be minimal. In contrast, during periods of dietary zinc deficiency when secretion of zinc by the pancreas and intestine is minimized, ZIP5 is removed from the cell surface, and the intestinal uptake of zinc is augmented by induction of ZIP4. |
| Databáze: | OpenAIRE |
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