The metal dependence of pyoverdine interactions with its outer membrane receptor FpvA
| Autor: | Gabrielle Zeder-Lutz, Franc Pattus, Agnès Hagège, Hervé Celia, Jason Greenwald |
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| Přispěvatelé: | Celia, Herve, Institut Gilbert-Laustriat : Biomolécules, Biotechnologie, Innovation Thérapeutique, Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Laboratoire de Spectrométrie de Masse BioOrganique [Strasbourg] (LSMBO), Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Cytoplasm
Siderophore Iron Biology 7. Clean energy Microbiology Metal 03 medical and health sciences chemistry.chemical_compound Fluorescence Resonance Energy Transfer [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Gene Regulation [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Chelation Surface plasmon resonance Molecular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology Manganese 0303 health sciences Pyoverdine 030306 microbiology Periplasmic space Surface Plasmon Resonance Culture Media Förster resonance energy transfer Biochemistry chemistry visual_art Periplasm Pseudomonas aeruginosa visual_art.visual_art_medium Bacterial outer membrane Oligopeptides Aluminum Bacterial Outer Membrane Proteins Protein Binding |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 2008, 190 (20), pp.6548-58 |
| ISSN: | 0021-9193 1098-5530 |
| Popis: | To acquire iron,Pseudomonas aeruginosasecretes the fluorescent siderophore pyoverdine (Pvd), which chelates iron and shuttles it into the cells via the specific outer membrane transporter FpvA. We studied the role of iron and other metals in the binding and transport of Pvd by FpvA and conclude that there is no significant affinity between FpvA and metal-free Pvd. We found that the fluorescent in vivo complex of iron-free FpvA-Pvd is in fact a complex with aluminum (FpvA-Pvd-Al) formed from trace aluminum in the growth medium. WhenPseudomonas aeruginosawas cultured in a medium that had been treated with a metal affinity resin, the in vivo formation of the FpvA-Pvd complex and the recycling of Pvd on FpvA were nearly abolished. The accumulation of Pvd in the periplasm ofPseudomonas aeruginosawas also reduced in the treated growth medium, while the addition of 1 μM AlCl3to the treated medium restored the effects of trace metals observed in standard growth medium. Using fluorescent resonance energy transfer and surface plasmon resonance techniques, the in vitro interactions between Pvd and detergent-solubilized FpvA were also shown to be metal dependent. We demonstrated that FpvA binds Pvd-Fe but not Pvd and that Pvd did not compete with Pvd-Fe for FpvA binding. In light of our finding that the Pvd-Al complex is transported across the outer membrane ofPseudomonas aeruginosa, a model for siderophore recognition based on a metal-induced conformation followed by redox selectivity for iron is discussed. |
| Databáze: | OpenAIRE |
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