Dyslexia-Associated Kiaa0319-Like Protein Interacts with Axon Guidance Receptor Nogo Receptor 1
| Autor: | Ming Wai Poon, Wan Hong Tsang, Ho Keung Ng, Sun-On Chan, Mary M.Y. Waye, Hiu Ming Li |
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| Rok vydání: | 2010 |
| Předmět: |
Candidate gene
Biopsy Nerve Tissue Proteins Receptors Cell Surface Biology Cytoplasmic Granules GPI-Linked Proteins Models Biological Protein–protein interaction Dyslexia Cellular and Molecular Neuroscience Nogo Receptor 1 mental disorders medicine Humans Immunoprecipitation 5-HT5A receptor Cells Cultured Cerebral Cortex Nuclear Proteins Colocalization Cell Biology General Medicine Human brain medicine.disease Immunohistochemistry Axons medicine.anatomical_structure Axon guidance Neuroscience Myelin Proteins Protein Binding |
| Zdroj: | Cellular and Molecular Neurobiology. 31:27-35 |
| ISSN: | 1573-6830 0272-4340 |
| DOI: | 10.1007/s10571-010-9549-1 |
| Popis: | To identify the putative interacting partners for Kiaa0319-like protein. KIAA0319-like, located near the dyslexia susceptibility locus, DYX8 in chromosome 1p34.3, has been suggested as a positional candidate for developmental dyslexia due to its homology with another gene, KIAA0319 which has been strongly established as a candidate gene for developmental dyslexia. Previous research has shown that a single marker, rs7523017 (P = 0.042) has been associated with developmental dyslexia by a Canadian group. There is little functional information about this gene and protein. In this article, we put forward further evidence that support Kiaa0319-like is a candidate for this disorder. A yeast-2-hybrid screen and co-immunopreciptiation assays were performed to find protein interacting partners of KIAA0319L. A human cortex immunohistochemistry assay was performed to show the colocalization of Kiaa0319-like and its specific interacting partner in cells. Nogo Receptor 1 (NgR1), an axon guidance receptor, was identified to have physical interactions with Kiaa0319-like protein. These two proteins interact predominantly in the cytoplasmic granules of cortical neurons in the human brain cortex. Based on this data, it can be concluded that Kiaa0319-like protein interacts with Nogo Receptor 1, supporting the idea that Kiaa0319-like protein participates in axon guidance. |
| Databáze: | OpenAIRE |
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