Expression Patterns of the slit Subfamily mRNA in Canine Malignant Mammary Tumors
| Autor: | Fumihito Ohashi, Mitsuru Kuwamura, Hideo Akiyoshi, Shingo Tsuyama, Shigeo Takenaka, Jyoji Yamate, Takaaki Sugiura, Yuchie Tanaka, Kihei Kubo, Toshihiko Tanno |
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| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty Molecular Sequence Data Mammary gland Gene Expression Mammary Neoplasms Animal Nerve Tissue Proteins Biology SLIT3 Dogs SLIT1 medicine SLIT2 Animals Dog Diseases RNA Messenger Cloning Molecular DNA Primers Glycoproteins Tube formation Mammary tumor Base Sequence General Veterinary Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis DNA Slit medicine.anatomical_structure Cancer research Leukocyte chemotaxis |
| Zdroj: | Journal of Veterinary Medical Science. 68:1173-1177 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.68.1173 |
| Popis: | Slit, a secreted protein, functions as a chemorepellent factor in axon guidance and neuronal migration and as an inhibitor in leukocyte chemotaxis. In humans, slit2 protein attracts endothelial cells and promotes tube formation in the tumor angiogenic mechanism. In this study, we cloned a part of the canine slit subfamily and examined the expression of slit subfamily mRNAs in 3 normal canine mammary glands and 11 mammary tumor samples by RT-PCR. The cloned part of the slit gene sequences showed high similarity to those of the human, mouse, and rat. The mRNAs were expressed at low levels in the normal mammary gland. The expression levels of slit1 mRNA were low in both the normal and tumor tissues. In contrast, the expression of slit2 mRNA increased in most of the malignant mammary tumors, and an increase in slit3 mRNA expression was observed in 2 of the malignant mixed tumors. These results suggest that the expression of slit2 plays an important role in tumor angiogenesis in canine mammary gland tumors and that slit2 can be a putative marker for malignancy diagnosis of these tumors. |
| Databáze: | OpenAIRE |
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