Special susceptibility to apoptosis of CD1a+ dendritic cell precursors differentiating from cord blood CD34+ progenitors
| Autor: | Jean Claude Gluckman, Micael Yagello, Bruno Canque, Sandrine Camus |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
Cell Survival CD14 T-Lymphocytes Stem cell factor Antigens CD34 Apoptosis Biology Antigens CD1 Antigens CD Humans Progenitor cell Cells Cultured Interleukin 3 Stem Cell Factor integumentary system Tumor Necrosis Factor-alpha Macrophages Cell Cycle Infant Newborn Granulocyte-Macrophage Colony-Stimulating Factor Membrane Proteins Cell Differentiation Cell Biology Dendritic cell Dendritic Cells Fetal Blood Flow Cytometry Hematopoietic Stem Cells Cell biology Hematopoiesis Haematopoiesis Kinetics Molecular Medicine Tumor necrosis factor alpha Lymphocyte Culture Test Mixed Cell Division Developmental Biology |
| Zdroj: | Stem cells (Dayton, Ohio). 16(3) |
| ISSN: | 1066-5099 |
| Popis: | We analyzed the effect of tumor necrosis factor (TNF)-alpha on the differentiation and viability of dendritic cells (DC) generated from cord blood CD34+ progenitors cultured for five days with GM-CSF, Flt-3 ligand (FL), and stem cell factor (SCF), and then with GM-CSF only [TNF(-) cultures]. Adding TNF-alpha from the start [TNF(+) cultures] potentiated progenitor cell proliferation and promoted early differentiation of CD1a+ DC precursors without affecting differentiation of CD14+ cells, which comprise bipotent precursors of DC and macrophages, nor of CD15+ granulocytic cells. Use of TNF-alpha was associated with increased cell mortality, which peaked on culture day 10 and mainly involved CD1a+ DC. Selective apoptosis of CD1a+ DC precursors was confirmed by showing that survival of day-7-sorted CD1a+CD14- cells from TNF(+) cultures was lower than that of CD1a-CD14+ cells. That similar findings were noted for sorted CD1a+CD14- cells of TNF(-) cultures, further cultured with GM-CSF without or with TNF-alpha, indicates that apoptosis of CD1a+ DC precursors was not induced by TNF-alpha. Apoptosis of CD1a+ DC precursors occurred after the cells had lost the capacity to incorporate bromodeoxyuridin. Finally, using higher GM-CSF concentrations or adding interleukin 3 (IL-3) improved viability of CD1a+ cells. Other cytokines, such as IL-4 and transforming growth factor (TGF)-beta1, were ineffective in this respect, though they promoted differentiation of CD1a+ DC. These results indicate that TNF-alpha promotes the differentiation of CD1a+ DC precursors, which display a high susceptibility to apoptosis that can be prevented by high concentrations of GM-CSF or use of IL-3, without affecting the differentiation of the CD14+ DC precursors. |
| Databáze: | OpenAIRE |
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