Metabolite profiling of pea roots in response to phosphate availability
| Autor: | Jérôme Laparre, Pascal Ludwiczak, Fabien Létisse, Virginie Puech-Pagès, Coline Balzergue, Jean-Charles Portais, Guillaume Bécard, Soizic Rochange |
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| Přispěvatelé: | Laboratoire de Recherche en Sciences Végétales (LRSV), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Symbiose mycorhizienne et Signalisation cellulaire, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Institut de pharmacologie et de biologie structurale (IPBS), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Evolution des Interactions Plantes-Microorganismes, Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Interactions Microbiennes dans la Rhizosphère et les Racines, Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Plant Science Biology Mass spectrometry Plant Roots Mass Spectrometry Phosphates Glomeromycota chemistry.chemical_compound Metabolomics Human fertilization Symbiosis Botany phosphorus Chromatography High Pressure Liquid 2. Zero hunger Principal Component Analysis Plant Extracts arbuscular mycorrhiza Peas metabolic profiling Discriminant Analysis 15. Life on land biology.organism_classification Phosphate Article Addendum chemistry Metabolite profiling Arbuscular mycorrhizal |
| Zdroj: | Plant Signaling and Behavior Plant Signaling and Behavior, Taylor & Francis, 2014, 6 (6), pp.837-839. ⟨10.4161/psb.6.6.15168⟩ Plant Signaling and Behavior, 2014, 6 (6), pp.837-839. ⟨10.4161/psb.6.6.15168⟩ |
| ISSN: | 1559-2316 1559-2324 |
| Popis: | International audience; The arbuscular mycorrhizal (AM) symbiosis is a mutualistic association between soil fungi (Glomeromycota) and roots of most plant species. A recent study showed that high phosphate fertilization could inhibit mycorrhizal colonization at a very early stage, before hyphopodium formation. The authors proposed that inhibiting and/or stimulatory compounds might be present in roots grown under high phosphate or low phosphate, respectively. To further address this question, we performed metabolite profiling analyses of extracts of pea roots grown under low and high phosphate concentrations. Ultra high performance liquid chromatography (UHPLC) was coupled with high resolution (HR) mass spectrometry (Q-TOF) and multivariate statistical analysis. This allowed the detection of 34 ions discriminating the two conditions. A majority (28 ions) were more abundant in roots grown under low phosphate concentration, and among them four were specific of this condition. The results suggest that the regulation of AM symbiosis by phosphate may involve the synthesis or accumulation of stimulatory compounds in roots grown under low phosphate. |
| Databáze: | OpenAIRE |
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