Evaluation of Cytokines as Robust Diagnostic Biomarkers for COVID-19 Detection
| Autor: | Álvaro Tamayo-Velasco, Paloma Cal-Sabater, María Heredia-Rodríguez, Marta Martín-Fernández, Pedro Martínez-Paz, José María Eiros, Eduardo Tamayo, Ignacio de la Fuente, José Pablo Miramontes-González, Rocío Aller, Hugo Gonzalo-Benito, Carlos Dueñas, Aida Fiz-López, Francisco Javier Álvarez, Esther Gómez-Sánchez, David Bernardo, María Jesús Peñarrubia-Ponce, Elisa Arribas-Rodríguez |
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| Přispěvatelé: | Instituto de Salud Carlos III, Consejo Superior de Investigaciones Científicas (España), Junta de Castilla y León, CSIC-UVA - Instituto de Biología y Genética Molecular (IBGM), Tamayo-Velasco, Álvaro, Álvarez, Francisco Javier, Fuente, Ignacio de la, Martín-Fernández, Marta, Eirós, José M., Martínez-Paz, Pedro, Miramontes González, José Pablo, Fiz-López, Aida, Arribas, Elisa, Cal-Sabater, Paloma de la, Dueñas-Gutiérrez, Carlos, Heredia-Rodríguez, María, Bernardo, David, Gómez-Sánchez, Esther, Tamayo-Velasco, Álvaro [0000-0001-6202-5420], Álvarez, Francisco Javier [0000-0002-7566-5678], Fuente, Ignacio de la [0000-0001-5381-820X], Martín-Fernández, Marta [0000-0003-2714-8120], Eirós, José M. [0000-0002-5357-6340], Martínez-Paz, Pedro [0000-0002-5772-8153], Miramontes González, José Pablo [0000-0002-2247-9679], Fiz-López, Aida [0000-0003-2052-7996], Arribas, Elisa [0000-0002-4470-075X], Cal-Sabater, Paloma de la [0000-0003-0267-794X], Dueñas-Gutiérrez, Carlos [0000-0002-8943-1231], Heredia-Rodríguez, María [0000-0003-3691-0252], Bernardo, David [0000-0002-2843-6696], Gómez-Sánchez, Esther [0000-0002-9457-1394] |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Medicine (miscellaneous) IP-10 Article law.invention 03 medical and health sciences 0302 clinical medicine Antigen law Internal medicine Diagnosis Validation medicine Diagnostic biomarker Polymerase chain reaction business.industry Diagnóstico COVID-19 Plasma levels COVID-19 (Enfermedad) 030104 developmental biology Biomarcadores 030220 oncology & carcinogenesis Cohort Medicine Biomarker (medicine) biomarker business Biomarkers |
| Zdroj: | UVaDOC. Repositorio Documental de la Universidad de Valladolid instname Journal of Personalized Medicine Volume 11 Issue 7 Journal of Personalized Medicine, Vol 11, Iss 681, p 681 (2021) Digital.CSIC. Repositorio Institucional del CSIC |
| Popis: | Antigen tests or polymerase chain reaction (PCR) amplification are currently COVID-19 diagnostic tools. However, developing complementary diagnosis tools is mandatory. Thus, we performed a plasma cytokine array in COVID-19 patients to identify novel diagnostic biomarkers. A discovery–validation study in two independent prospective cohorts was performed. The discovery cohort included 136 COVID-19 and non-COVID-19 patients recruited consecutively from 24 March to 11 April 2020. Forty-five cytokines’ quantification by the MAGPIX system (Luminex Corp., Austin, TX, USA) was performed in plasma samples. The validation cohort included 117 patients recruited consecutively from 15 to 25 April 2020 for validating results by ELISA. COVID-19 patients showed different levels of multiple cytokines compared to non-COVID-19 patients. A single chemokine, IP-10, accurately identified COVID-19 patients who required hospital admission (AUC: 0.962 95%CI (0.933–0.992) p < 0.001)). The results were validated in an independent cohort by multivariable analysis (OR: 25.573 95%CI (8.127–80.469) 0.001) and AUROC (AUC: 0.900 95%CI (0.846–0.954) 0.001). Moreover, showing IP-10 plasma levels over 173.35 pg/mL identified COVID-19 with higher sensitivity (86.20%) than the first SARS-CoV-2 PCR. Our discover–validation study identified IP-10 as a robust biomarker in clinical practice for COVID-19 diagnosis at hospital. Therefore, IP-10 could be used as a complementary tool in clinical practice, especially in emergency departments. |
| Databáze: | OpenAIRE |
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