Materials for enhancing cell adhesion by immobilization of cell-adhesive peptide
| Autor: | Yoshihiro Ito, Yukio Imanishi, Masako Kajihara |
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| Rok vydání: | 1991 |
| Předmět: |
Materials science
Molecular Sequence Data Biomedical Engineering Peptide Biomaterials chemistry.chemical_compound Mice Polymer chemistry Cell Adhesion Animals Amino Acid Sequence Cell adhesion Cells Cultured Acrylic acid Carbodiimide chemistry.chemical_classification biology Temperature Biomaterial Adhesion Hydrogen-Ion Concentration Fibronectins Fibronectin chemistry Chemical engineering embryonic structures biology.protein Polystyrenes Spectrophotometry Ultraviolet Polystyrene Peptides Cell Division |
| Zdroj: | Journal of biomedical materials research. 25(11) |
| ISSN: | 0021-9304 |
| Popis: | Materials to enhance cell adhesion were synthesized by surface integration of peptide, Arg-Gly-Asp-Ser(RGDS), which is an active-site sequence of cell-adhesive proteins. Polystyrene film was glow-discharged and graft-copolymerized with acrylic acid. Then the peptide was immobilized to the poly(acrylic acid) grafts by using water-soluble carbodiimide. The cell-adhesive activity of the RGDS-immobilized film increased with increasing amount of immobilized peptide, and approached the activity of fibronectin(FN)-immobilized film. The RGDS-immobilized film was more stable against heat treatment and pH variation than the FN-immobilized film. In addition, the RGDS-immobilized film enhanced cell growth more strongly than the FN-immobilized film. |
| Databáze: | OpenAIRE |
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