Overexpression of RAGE Contributes to Cigarette Smoke-Induced Nitric Oxide Generation in COPD
| Autor: | Chun Wan, Dan Xu, Fuqiang Wen, Lingli Guo, Yongchun Shen, Ting Yang, Zenglin Liao, Tao Wang, Lei Chen |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine medicine.medical_specialty endocrine system diseases Receptor for Advanced Glycation End Products Nitric Oxide Nitric oxide Proinflammatory cytokine RAGE (receptor) Pulmonary Disease Chronic Obstructive chemistry.chemical_compound Downregulation and upregulation Glycation Internal medicine medicine Humans Receptors Immunologic Receptor Lung Cells Cultured Aged COPD biology business.industry Smoking Transcription Factor RelA nutritional and metabolic diseases Middle Aged medicine.disease Glutathione Up-Regulation respiratory tract diseases Nitric oxide synthase Endocrinology chemistry Case-Control Studies cardiovascular system biology.protein Female Nitric Oxide Synthase business human activities Signal Transduction |
| Zdroj: | Lung. 192:267-275 |
| ISSN: | 1432-1750 0341-2040 |
| Popis: | Receptor for advanced glycation end products (RAGE), a multiple-ligands receptor, is implicated in chronic obstructive pulmonary disease (COPD). This study was designed to investigate the potential role of RAGE in nitric oxide (NO) generation, an endogenous marker of nitrosative stress in COPD. Lung tissues from COPD patients were used to describe the relationship between RAGE expression and NO level. RAGE expression was assessed by immunohistochemistry, western blot, and ELISA. Human bronchial epithelial cells (16HBE) were cultured with cigarette smoke extract (CSE). Neutralizing antibody against RAGE was used to detect the role of RAGE in CSE-induced NO generation by 16HBE cells. Compared with nonsmoker controls, overexpression of RAGE was significantly detected in COPD smokers (p |
| Databáze: | OpenAIRE |
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