Isolation and characterization of diuron-degrading bacteria from lotic surface water
| Autor: | Stéphane Pesce, Jacques Bohatier, Martine Sancelme, Pascale Besse-Hoggan, Isabelle Batisson |
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| Přispěvatelé: | Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Centre National de la Recherche Scientifique (CNRS), Synthèse et étude de systèmes à intêret biologique (SEESIB), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Cellulaire (LBC), Université d'Auvergne - Clermont-Ferrand I (UdA), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
MESH: Sequence Analysis
DNA Culture MESH: Water Pollutants 010501 environmental sciences 01 natural sciences Enrichment culture Gene MESH: Biodegradation Environmental Strain RNA Ribosomal 16S Agar Water Pollutants MESH: Phylogeny Phylogeny Variable region 0303 health sciences Ribosomal DNA Ecology biology Reproduction Pseudomonas Surface water Temperature gradient 6. Clean water Community structure Stenotrophomonas MESH: RNA Ribosomal 16S Biodegradation Environmental MESH: Diuron Electrophoresis Polyacrylamide Gel Pseudomonadaceae [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology Temperature gradient gel electrophoresis DNA Bacterial food.ingredient Characterization Molecular Sequence Data Soil Science Microbiology Isolation MESH: Rivers Agar plate 03 medical and health sciences food Rivers Microbial community Colony Pseudomonadales Hybridization Ecology Evolution Behavior and Systematics 030304 developmental biology 0105 earth and related environmental sciences MESH: Herbicides MESH: Molecular Sequence Data Bacteria MESH: Stenotrophomonas Herbicides MESH: Pseudomonas Sequence Analysis DNA biology.organism_classification Gel electrophoresis MESH: DNA Bacterial Culture Media Diuron MESH: Culture Media Geographic distribution MESH: Electrophoresis Polyacrylamide Gel |
| Zdroj: | Microbial ecology Microbial ecology, 2007, 54 (4), pp.761-70. ⟨10.1007/s00248-007-9241-2⟩ Microbial Ecology Microbial Ecology, Springer Verlag, 2007, 54 (4), pp.761-70. ⟨10.1007/s00248-007-9241-2⟩ |
| ISSN: | 0095-3628 1432-184X |
| DOI: | 10.1007/s00248-007-9241-2⟩ |
| Popis: | International audience; The bacterial community structure of a diuron-degrading enrichment culture from lotic surface water samples was analyzed and the diuron-degrading strains were selected using a series of techniques combining temporal temperature gradient gel electrophoresis (TTGE) of 16 S rDNA gene V1-V3 variable regions, isolation of strains on agar plates, colony hybridization methods, and biodegradation assays. The TTGE fingerprints revealed that diuron had a strong impact on bacterial community structure and highlighted both diuron-sensitive and diuron-adapted bacterial strains. Two bacterial strains, designated IB78 and IB93 and identified as belonging to Pseudomonas sp. and Stenotrophomonas sp., were isolated and shown to degrade diuron in pure resting cells in a first-order kinetic reaction during the first 24 h of incubation with no 3,4-DCA detected. The percentages of degradation varied from 25% to 60% for IB78 and 20% to 65% for IB93 and for a diuron concentration range from 20 mg/L to 2 mg/L, respectively. It is interesting to note that diuron was less degraded by single isolates than by mixed resting cells, thereby underlining a cumulative effect between these two strains. To the best of our knowledge, this is the first report of diuron-degrading strains isolated from lotic surface water. |
| Databáze: | OpenAIRE |
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