SLA-1 Genetic Diversity in Pigs: Extensive Analysis of Copy Number Variation, Heterozygosity, Expression, and Breed Specificity
| Autor: | Jin-Hoi Kim, Hojun Choi, Byeongyong Ahn, Chankyu Park, Minh Thong Le, Kwonho Hong, Hye Jeong Lee, Van Chanh Quy Le, Chak-Sum Ho, Hyuk Song |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Agricultural genetics Heterozygote DNA Copy Number Variations Swine Gene Expression lcsh:Medicine Biology Breeding Article Cell Line Loss of heterozygosity 03 medical and health sciences 0302 clinical medicine Gene duplication Genetic variation Immunogenetics Animals Copy-number variation RNA Messenger Allele lcsh:Science Alleles Genetics Genetic diversity Multidisciplinary Histocompatibility Testing Haplotype Histocompatibility Antigens Class I fungi lcsh:R Genetic Variation High-Throughput Nucleotide Sequencing 030104 developmental biology Haplotypes lcsh:Q Purebred 030215 immunology |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-57712-5 |
| Popis: | Swine leukocyte antigens play indispensable roles in immune responses by recognizing a large number of foreign antigens and thus, their genetic diversity plays a critical role in their functions. In this study, we developed a new high-resolution typing method for pig SLA-1 and successfully typed 307 individuals from diverse genetic backgrounds including 11 pure breeds, 1 cross bred, and 12 cell lines. We identified a total of 52 alleles including 18 novel alleles and 9 SLA-1 duplication haplotypes, including 4 new haplotypes. We observed significant differences in the distribution of SLA-1 alleles among the different pig breeds, including the breed specific alleles. SLA-1 duplication was observed in 33% of the chromosomes and was especially high in the biomedical model breeds such as SNU (100%) and NIH (76%) miniature pigs. Our analysis showed that SLA-1 duplication is associated with the increased level of SLA-1 mRNA expression in porcine cells compared to that of the single copy haplotype. Therefore, we provide here the results of the most extensive genetic analysis on pig SLA-1. |
| Databáze: | OpenAIRE |
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