Lung Perfusion Imaging with Monosized Biodegradable Microspheres
| Autor: | Boris Stoeber, Urs O. Häfeli, N Renée Labiris, Ripen Misri, Mehrdad Bokharaei, Philipp Elischer, Katayoun Saatchi |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Biodistribution Polymers and Plastics Stereochemistry Bioengineering Scintigraphy Microsphere Biomaterials Mice In vivo Technetium-99 Materials Chemistry medicine Animals Humans Tissue Distribution Chelation Microparticle Lung Tomography Emission-Computed Single-Photon medicine.diagnostic_test Chemistry Microspheres Mice Inbred C57BL medicine.anatomical_structure Microscopy Electron Scanning Female Biomedical engineering |
| Zdroj: | Biomacromolecules. 11:561-567 |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/bm9010722 |
| Popis: | After intravenous injection, particles larger than red blood cells will be trapped in the first capillary bed that they encounter. This is the principle of lung perfusion imaging in nuclear medicine, where macroaggregated albumin (MAA) is radiolabeled with (99m)Tc, infused into a patient's arm vein, and then imaged with gamma scintigraphy. Our aim was to evaluate if monosized microspheres could replace (99m)Tc-MAA. Biodegradable poly(L-lactide) microspheres containing chelating bis(picolylamine) end groups were prepared by a flow focusing method on a microfluidic glass chip and were of highly homogeneous size (9.0 +/- 0.4 microm). The microspheres were radiolabeled with [(99m)Tc(H(2)O)(3)(CO)(3)](+) and then evaluated in mice for lung perfusion imaging. Fifteen minutes after injection, 79.6 +/- 3.8% of the injected activity was trapped in the lungs of mice. Monosized biodegradable radioactive microspheres are, thus, appropriate lung perfusion imaging agents. Other sizes of these highly uniform microspheres have the potential to improve diagnostic and therapeutic approaches in diverse areas of medicine. |
| Databáze: | OpenAIRE |
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