3D Printable, Modified Trephine Designs for Consistent Anterior Lamellar Keratectomy Wounds in Rabbits
| Autor: | Fang Chen, Xin Xia, Ignacio Blanco Blanco Varela, Spencer Q Montague-Alamin, David C Mundy, David Buickians, Caitlin M Logan, Peter Le, David Myung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Swine Surgical Wound Article Cornea Corneal Transplantation 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Ophthalmology medicine Animals Lamellar structure Polytetrafluoroethylene business.industry fungi food and beverages Rabbit (nuclear engineering) Equipment Design Surgical training Sensory Systems Disease Models Animal Resins Synthetic Trephine Printing Three-Dimensional 030221 ophthalmology & optometry Rabbits business 030217 neurology & neurosurgery Tomography Optical Coherence |
| Zdroj: | Curr Eye Res |
| Popis: | PURPOSE: Our goal is to develop a low-cost tool that can be used to create consistent, partial thickness defects in rabbit and other large animals with minimal surgical training and that can facilitate pre-clinical testing of lamellar and in situ-forming biosynthetic matrix materials for corneal repair. MATERIALS & METHODS: In this study, three modified trephines were designed to create deep corneal wound defects with consistent depth in large animals. The modified trephines incorporated either 3D printed parts made from photopolymerizable resins, or custom-cut commercially available Teflon sheets. Wound defects were imaged with optical coherence tomography (OCT), and the depth was analyzed based on the OCT images. RESULTS: The results revealed that an inner-stopper guard trephine had the best performance in creating consistent and precise wound defect depth compared to modified vacuum trephine and custom guard vacuum trephine. A 75% ± 10% cut of the cornea was achieved with inner-stopper guard trephine. The wound defect depth by inner-stopper guard trephine was independent on the corneal thickness or size of the globes. Although the cut depth by inner-stopper guard trephine relied on the experiences of users, the consistency (standard deviation) of the depth was independent on the experience of users. CONCLUSIONS: Our studies provided three cost-efficient animal trephines that could create consistent corneal wound depth by lab researchers without extensive training in keratectomy. |
| Databáze: | OpenAIRE |
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