Biosurfactant production by Serratia marcescens SS-1 and its isogenic strain SMdeltaR defective in SpnR, a quorum-sensing LuxR family protein
| Autor: | Yu Hong Wei, Jo Shu Chang, Shan-Yu Chen, Mao Song Yeh, Hsin Chih Lai |
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| Rok vydání: | 2004 |
| Předmět: |
biology
Strain (chemistry) Bioengineering General Medicine biology.organism_classification Applied Microbiology and Biotechnology Enterobacteriaceae Microbiology Kerosene Repressor Proteins Diesel fuel Quorum sensing Surface-Active Agents Pulmonary surfactant Species Specificity Serratia marcescens Extracellular Trans-Activators Surface Tension Emulsions Genetic Engineering Bacteria Gasoline Biotechnology |
| Zdroj: | Biotechnology letters. 26(10) |
| ISSN: | 0141-5492 |
| Popis: | Serratia marcescens SS-1 and its SpnR-defective isogenic mutant, SMdeltaR, produced an extracellular surfactant able to decrease surface tension of water from 72 to 37 dyne cm(-1) (SMdeltaR strain) and to 45 dyne cm(-1) (SS-1 strain). The biosurfactant also emulsified kerosene and diesel with a maximum emulsion index of 77% (diesel and kerosene) for the SMdeltaR strain, and 72% (kerosene) and 40% (diesel) for the SS-1 strain. Deletion of spnR gene appeared to enhance biosurfactant production. Model simulations suggest that biosurfactant production by the two strains was growth-associated. The SMdeltaR strain had a yield coefficient of 22-32% g dry cell(-1), which is 32-50% higher than that of the SS-1 strain. |
| Databáze: | OpenAIRE |
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