Lymphocyte production of gamma-interferon as a test for non-tuberculous mycobacterial lymphadenitis in childhood
| Autor: | D. M. Roberton, C. S. Hosking, Patricia M. Davidson, P. R. Wood, L. Creati |
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| Rok vydání: | 1993 |
| Předmět: |
Non tuberculous mycobacterial
Male Pathology medicine.medical_specialty Adolescent Mycobacterium Infections Nontuberculous Tuberculin Interferon-gamma Cervical lymphadenopathy Gamma interferon Medicine Humans Interferon gamma Lymphocytes Prospective Studies Prospective cohort study Child Lymphatic Diseases Whole blood Mycobacterium avium-intracellulare Infection business.industry Infant Mycobacterium scrofulaceum Lymphocyte production Antibodies Bacterial In vitro Child Preschool Pediatrics Perinatology and Child Health Immunology Female medicine.symptom business Neck medicine.drug Mycobacterium avium |
| Zdroj: | European journal of pediatrics. 152(1) |
| ISSN: | 0340-6199 |
| Popis: | Infection with Mycobacterium avium, M. intracellulare and M. scrofulaceum (MAIS) organisms in normal children may result in cervical lymphadenopathy. There is a poor response to anti-tuberculous therapy and surgical excision of infected nodes is necessary. The diagnosis therefore requires consideration in children with cervical lymphadenopathy. A simple in vitro assay is described which may be useful for diagnosis prior to excision. Whole blood is cultured with M. avium purified protein derivative. After 24 h the plasma is removed and the concentration of gamma interferon (IFN-gamma) quantified by a radioimmunometric assay. In a prospective study of 38 children with neck lesions, 16 showed MAIS organisms isolated from cultures of excised tissue. The level of IFN-gamma produced by peripheral blood lymphocytes from children in the MAIS group was significantly greater when compared with children having operations for neck lesions that were not due to MAIS organisms (P0.001). |
| Databáze: | OpenAIRE |
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