Von Willebrand Factor: Multimeric Structure and Functional Activity in Patients With Atrial Fibrillation With and Without Oral Anticoagulation
| Autor: | Sandra Lopez-Castaneda, Martha Eva Viveros-Sandoval, Daniel Godínez-Hernández, Carlos Arean, Ignacio Valencia-Hernández |
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| Rok vydání: | 2017 |
| Předmět: |
Male
congenital hereditary and neonatal diseases and abnormalities medicine.medical_specialty Administration Oral 030204 cardiovascular system & hematology 03 medical and health sciences 0302 clinical medicine Von Willebrand factor Western blot Antigen Risk Factors hemic and lymphatic diseases Internal medicine Atrial Fibrillation von Willebrand Factor Blood plasma medicine Humans Stroke Aged chemistry.chemical_classification biology medicine.diagnostic_test business.industry Anticoagulants Atrial fibrillation Original Articles Hematology General Medicine medicine.disease ADAMTS13 Endocrinology chemistry cardiovascular system biology.protein Female Glycoprotein business 030217 neurology & neurosurgery circulatory and respiratory physiology |
| Zdroj: | Clinical and Applied Thrombosis/Hemostasis. 24:647-654 |
| ISSN: | 1938-2723 1076-0296 |
| DOI: | 10.1177/1076029617711803 |
| Popis: | von Willebrand factor (vWF) is a multimeric glycoprotein present in blood plasma. It is synthesized in megakaryocytes and endothelial cells, secreted into circulation in the form of high-molecular-weight multimers (HMWMs), and cleaved into shorter, less active multimers by ADAMTS13. It is essential for platelet adhesion and aggregation. Previous studies have investigated the relationship between vWF levels and thromboembolic events with little regard to vWF multimeric structure. Patients with atrial fibrillation (AF) exhibit higher plasma vWF and lower ADAMTS13 levels. One hundred seven patients with AF, 51 anticoagulated and 56 nonanticoagulated, were eligible for the study. Plasma samples were analyzed for vWF antigen, vWF activity, and ADAMTS13; vWF multimers were analyzed by Western blot in 1% to 1.3% sodium dodecyl sulfate agarose gel electrophoresis. Patients with AF without oral anticoagulation (OAC) had significantly higher vWF plasma levels (154.00 [75-201] UI/dL) and vWF activity (60.00% [20%-210%]) compared to patients with OAC (133.50 [90-192] UI/dL, P = |
| Databáze: | OpenAIRE |
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