The Role of B/T Costimulatory Signals in the Immunopotentiating Activity of Neisserial Porin
| Autor: | Milan S. Blake, Fiona G. Mackinnon, Mohammed H. Sayegh, Yu Ho, Francis Michon, Anil Chandraker, Lee M. Wetzler |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Immunoconjugates
Time Factors medicine.drug_class T-Lymphocytes T cell Porins Neisseria meningitidis Monoclonal antibody complex mixtures Immunoglobulin G Microbiology Abatacept Mice Immune system stomatognathic system Antigen Antigens CD medicine Animals Immunology and Allergy CTLA-4 Antigen Immunization Schedule Mice Inbred C3H CD40 biology Immunogenicity Polysaccharides Bacterial Antibodies Monoclonal biochemical phenomena metabolism and nutrition Antigens Differentiation Neisseria gonorrhoeae stomatognathic diseases Infectious Diseases medicine.anatomical_structure Immunoglobulin M Antibody Formation Bacterial Vaccines Immunology biology.protein bacteria |
| Zdroj: | The Journal of Infectious Diseases. 180:755-761 |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | A T cell-dependent immune response to group C meningococcal capsular polysaccharide (CPS) can be elicited when CPS is conjugated to the class 3 neisserial porin (CPS-porin). Treatment of CPS-porin-immunized mice with B7-2 blocking monoclonal antibody (MAb) caused a dramatic reduction in the CPS-specific IgG response, treatment with anti-B7-1 MAb had no effect, and concurrent blockade of B7-1 and B7-2 resulted in a synergistic abrogation of the CPS-specific IgG response while the CPS IgM response was unaffected. Anti-CD40L MAb treatment caused a significant reduction of both CPS-specific IgG and IgM levels. In contrast, blockade of CTLA4 interactions resulted in increases in both CPS IgG and IgM responses in CPS-porin-immunized mice. These data support the hypothesis that the ability of neisserial porins to improve the immune response to poorly immunogenic antigens (e.g., polysaccharides) is related to porin-induced increases in B7-2 expression on antigen-presenting cells and enhanced B/T cell interactions. |
| Databáze: | OpenAIRE |
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