Icariin induces the growth, migration and osteoblastic differentiation of human periodontal ligament fibroblasts by inhibiting Toll-like receptor 4 and NF-κB p65 phosphorylation
| Autor: | Xue-Yang Liu, De-Bao Jing, Hai-Jiang Liu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Cell Survival Periodontal Ligament growth Apoptosis migration Biochemistry Bone morphogenetic protein 2 NF-κB Extracellular matrix 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Osteoprotegerin Cell Movement Osteogenesis Genetics Humans Phosphorylation Receptor Molecular Biology Cells Cultured Cell Proliferation Flavonoids Osteoblasts Oncogene Chemistry NF-kappa B Cell Differentiation TLR-4 Articles Fibroblasts Cell biology Toll-Like Receptor 4 Protein Transport 030104 developmental biology Oncology 030220 oncology & carcinogenesis Molecular Medicine Signal transduction osteoblastic differentiation Icariin Signal Transduction |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | The proliferation, migration and differentiation capacities of human periodontal ligament fibroblasts (HPDLCs) are important for the treatment of periodontal diseases. The aim of the present study was to investigate whether icariin could promote these abilities in HPDLCs, and explore the cellular mechanisms therein. The results indicated that icarrin markedly blocked apoptosis, and increased the viability and migration of HPDLCs, particularly at the concentrations of 20 and 50 µM. In addition, icariin significantly promoted HPDLCs to synthesize extracellular matrix, which was reflected by the decreased expression of matrix matalloproteinase-1 and increased expression of tissue inhibitor of metalloproteinase-1. Furthermore, the levels of bone morphogenetic protein 2, collagen I, osteoprotegerin and alkaline phosphatase were markedly elevated by icariin, indicating that icariin was able to promote the osteogenic differentiation capability of HPDLCs. Icariin also inactivated the Toll-like receptor 4 (TLR)-4/nuclear factor (NF)-κB signaling pathway by suppressing the expression levels of TLR-4 and phosphorylated p65, and by blocking p65 nuclear translocation. These results suggested that icarrin increased the survival, migration and osteoblastic differentiation of HPDLCs by inhibiting the TLR-4/NF-κB signaling pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|