Selenium nanoparticles reduce glucose metabolism and promote apoptosis of glioma cells through reactive oxygen species-dependent manner
| Autor: | Xinlin Luo, Binchu Xu, Nan Zhou, Gengqiang Ling, Jian Guo, Qingchang Liu, Qingping Zhang, Juncheng Luo, Xinjie Ning |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Glucose uptake chemistry.chemical_element Apoptosis Selenium 03 medical and health sciences 0302 clinical medicine Annexin Cell Line Tumor Glioma medicine Humans MTT assay chemistry.chemical_classification Reactive oxygen species General Neuroscience medicine.disease Glucose 030104 developmental biology chemistry Cancer research Nanoparticles Reactive Oxygen Species 030217 neurology & neurosurgery Pyruvate kinase |
| Zdroj: | NeuroReport. 31:226-234 |
| ISSN: | 0959-4965 |
| Popis: | Gliomas are the most common, malignant, and lethal tumors in adults. Furthermore, gliomas are highly resistant to current chemotherapeutic drugs. Thus, new effective anticancer drugs for glioma are urgently needed. Selenium nanoparticles have been reported to have potent anti-tumor activity, although the specific mechanism is not fully understood. This study aimed to test the anti-tumor effect of selenium nanoparticles and its mechanism. We used selenium nanoparticles to treat commercial glioma cell lines, and patient-derived glioma cells, and then used the MTT assay to determine selenium nanoparticles effect against these. Apoptotic cell death was determined by annexin V-Fluos staining kit. Glucose uptake, lactate, and adenosine triphosphate production, together with hexokinase 2 and pyruvate kinase activities were measured to determine the glucose metabolism level. Reactive oxygen species production was tested using 2',7'-dichlorodihydrofluorescein diacetate. Our results showed that selenium nanoparticles had a potent cytotoxic effect in glioma cells, regardless of whether they were drug-resistant or not, whereas it showed less toxic effect in normal healthy cells. Further tests showed that selenium nanoparticles treatment leads to apoptotic cell death enhancement and glucose metabolism reduction, and this process was in a reactive oxygen species pathway-dependent manner. These results may provide a novel direction for glioma therapy in the future. |
| Databáze: | OpenAIRE |
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