An Unusual Radiation-induced G2Arrest in the Zygote of the BALB/c Mouse Strain
| Autor: | S. Grinfeld, P. Jacquet |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Time Factors
BALB/c Mouse Cell Survival Zygote Biology Andrology Mice chemistry.chemical_compound Species Specificity Pregnancy Animals reproductive and urinary physiology Cell Nucleus Mice Inbred BALB C Strain (chemistry) DNA synthesis Cell Cycle Embryo DNA General Medicine Cell cycle chemistry embryonic structures Immunology Female G2 arrest Cell Division |
| Zdroj: | International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine. 51:353-363 |
| ISSN: | 0020-7616 |
| DOI: | 10.1080/09553008714550821 |
| Popis: | Female mice of the BALB/c strain were superovulated, mated with males of the same strain, and irradiated with 1 Gy of X-rays at hourly intervals during the first cell cycle of the embryos. Two types of effects were found in the embryos, depending on the time of X-irradiation. When irradiation was delivered between 14 and 21 h after human chorionic gonadotrophin (hCG) injection, cultured two-cell embryos developed normally up to the morula stage, where a high mortality occurred. On the other hand, when irradiation was given between 17 and 24 h after hCG injection, a high proportion of the eggs was unable to cleave and remained blocked at the one-cell stage. Cytofluorometric analysis of the pronuclear DNA content of uncleaved zygotes showed that DNA synthesis was unaffected by X-irradiation, and that they were blocked in G2 phase of the first cell cycle. Similar studies on other strains, as well as reciprocal crosses between BALB/c and F1(female BALB/c X male C57 BLACK) mice showed that the 'one-cell block' is determined by the maternal genotype and results most probably from a direct action of X-rays on a radiosensitive cytoplasmic factor necessary for the first embryonic cell division, and appearing 17 h after hCG injection. A high proportion of blocked zygotes (30-40 per cent) recovered partially, cleaved with a delay of about 20 h, and died soon after, almost none of them being able to reach the blastocyst stage. At the time of maximum radiosensitivity, the LD50 for development up to the blastocyst stage was 0.95 Gy. |
| Databáze: | OpenAIRE |
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