Polyphenols Extracted from Chinese Hickory (Carya Cathayensis) Promote Apoptosis and Inhibit Proliferation through the p53-Dependent Intrinsic and HIF-1α-VEGF Pathways in Ovarian Cancer Cells
| Autor: | Yi Charlie Chen, Zhiping He, Shaozhen Wu, Ashley Booth, Gary O. Rankin, Ivan Martinez, Lin Ju |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
p53
endocrine system diseases lcsh:Technology lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine PTEN General Materials Science Viability assay Instrumentation lcsh:QH301-705.5 030304 developmental biology Fluid Flow and Transfer Processes 0303 health sciences biology Cell growth lcsh:T Process Chemistry and Technology General Engineering apoptosis Cancer medicine.disease lcsh:QC1-999 female genital diseases and pregnancy complications Computer Science Applications Vascular endothelial growth factor Chinese hickory polyphenol ovarian cancer chemistry lcsh:Biology (General) lcsh:QD1-999 Apoptosis lcsh:TA1-2040 030220 oncology & carcinogenesis Cancer cell Cancer research biology.protein Ovarian cancer lcsh:Engineering (General). Civil engineering (General) lcsh:Physics |
| Zdroj: | Applied Sciences Volume 10 Issue 23 Applied Sciences, Vol 10, Iss 8615, p 8615 (2020) |
| ISSN: | 2076-3417 |
| DOI: | 10.3390/app10238615 |
| Popis: | Ovarian cancer is the second most common gynecologic cancer with an estimated 13,940 mortalities across the United States in 2020. Natural polyphenols have been shown to double the survival time of some cancer patients due to their anticancer properties. Therefore, the effect of polyphenols extracted from Chinese hickory seed skin Carya cathayensis (CHSP) on ovarian cancer was investigated in the present study. Cell viability results showed that CHSP is more effective in inhibiting ovarian cancer cells than normal ovarian cells, with the IC50 value for inhibition of cell proliferation of Ovarian cancer cells (OVCAR-3) being 10.33 ± 0.166 µ g/mL for a 24 h treatment. Flow cytometry results showed that the apoptosis rate was significantly increased to 44.21% after 24 h treatment with 20 µ g/mL of CHSP. Western blot analysis showed that CHSP induced apoptosis of ovarian cancer cells through a p53-dependent intrinsic pathway. Compared with control values, levels of VEGF excreted by OVCAR-3 cancer cells were reduced to 7.87% with a 40 µ g/mL CHSP treatment. Consistent with our previous reports, CHSP inhibits vascular endothelial growth factor (VEGF) secretion by regulating the HIF-1&alpha VEGF pathway. In addition, we also found that the inhibitory effect of CHSP on ovarian cancer is related to the up-regulation of Phosphatase and tension homolog (PTEN) and down-regulation of nuclear factor kappa-B (NF-kappa B). These findings provide some evidence of the anti-ovarian cancer properties of CHSP and support the polyphenols as potential candidates for ovarian cancer adjuvant therapy. |
| Databáze: | OpenAIRE |
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